An extraction method for sargassum horneri polyphenol is characterized by comprising: A, taking sargassum horneri as a raw material, crushing sargassum horneri, and sieving by using a 25-35 mesh sieve, so as to obtain a uniform sargassum horneri powder; B, accurately weighing a certain amount of sargassum horneri powder, adding an organic solvent aqueous solution with the concentration of 40%-90% according to the material-solution ratio of 1:9-11 (w / v), and fully uniformly mixing and immersing; C, immersing for 10-15 min, and using a supersonic-wave cell crushing machine to crush for 5-10 min to perform extraction of polyphenol; D, performing centrifugal processing on the extraction liquid, and controlling the processing technology of 3500-4500 rpm and 4-6 min; E, repeatedly extracting for 4-6 times, and merging the supernatants; and F, performing 45 DEG C evaporation concentration on the supernatant by a rotary evaporator, and then performing vacuum freeze drying, so as to obtain the sargassum horneri polyphenol extractive. The extraction method overcomes long time, high temperature, low extraction rate and other adverse effects of a conventional method of using water to extract polyphenol, and is relatively simple and time-saving and low in cost, and the extracted sargassum horneri polyphenol product is high in content and relatively strong in cell antioxidation performance.