The invention provides a flow-type electroporation device. The device includes a pipe body, a first electrode, a second electrode, a support for fixing the first electrode, and a support for fixing the second electrode; and the device is characterized in that pipe openings of two ends of the pipe body are provided with grooves, the support for fixing the first electrode and the support for fixingthe second electrode are respectively arranged in the grooves, the shapes of the first electrode and the second electrode are the same as the shape of the cross section of the middle part of the pipebody, the pipe body forms a cavity with the first electrode and the second electrode, a gap is arranged between the support for fixing the first electrode and the support for fixing the second electrode, and a liquid enters and/or flows out of the cavity through the gap. The flow-type electroporation device provided by the invention can form an electroporation only when the cavity is filled with the liquid, ensures the same time for the cell electroporation, and improves the cell processing capacity while improving the electroporation efficiency.