The present application relates to the field of
medicine and
health food evaluation, in order to solve the problem of lacking the method for evaluating the
appetite regulating effect of
medicine and
health food in the prior art, a method for evaluating the
appetite regulating effect of
medicine and
health food is provided, different concentrations of the sample to be tested are given to
Nile red enteric labeled
zebrafish by water
dissolution, and the
zebrafish are cultured for 24-36 h, a
normal control group is set at the same time, the intestinal
fluorescence intensity of the
zebrafish in each group is analyzed, the sample to be tested is given to the zebrafish by water
dissolution, and the zebrafish are cultured for 48-72 h, a
normal control group is set at the same time, egg
yolk powder is fed for 1-3 h, part of the zebrafish in each group are selected, the sum of the intestinal
opacity and the
tail blood vessel staining intensity of the zebrafish are analyzed, the remaining zebrafish continue to be cultured for 24-48 h, and the intestinal short-chain
fatty acid fluorescence intensity and the expression of
appetite inhibiting genes and appetite promoting genes of the zebrafish are analyzed. The method can quantitatively evaluate the appetite regulating effect of medicine and health food, and the method steps are simple and time-saving.