The invention discloses a miniature pipe jacking construction method for ecological comprehensive pipe gallery engineering, which comprises the following construction steps: step 1, mounting and debugging a miniature pipe jacking machine, step 2, orienting a laser theodolite, step 3, jacking a pilot pipe, step 4, jacking an unearthed outer pipe, step 5, jacking a spiral cutter head, step 6, jacking a reinforced concrete pipe, and step 7, dismounting equipment. In the pipe jacking construction process, each section of pilot pipe and each section of unearthed outer pipe are sequentially dismantled in the receiving well, and the spiral cutter head, the pipe jacking machine table and the machine table base are dismantled in the working well. The miniature pipe jacking construction method for the ecological comprehensive pipe gallery project is easy to operate, high in pipe jacking efficiency, quite convenient and rapid to install and detach, good in pipe jacking accuracy and safety and suitable for rapid construction of urban ecological comprehensive pipe galleries.