The invention provides a heating device applied to a scanning electron microscope. The device comprises a sample crucible, a heating core ceramic, an electric heating wire and a heat radiation shielding structure, wherein the sample crucible is placed on an upper end of the heating core ceramic, the electric heating wire is wound around a surface of the heating core ceramic in a double helical manner, the heating core ceramic is heated, and the electric heating wire is made of a doped tungsten filament; the heat radiation shielding structure comprises an internal stainless steel shielding layer, a secondary external stainless steel shielding layer, an external stainless steel shielding layer, an upper stainless steel shielding layer, a secondary upper stainless steel shielding layer and a shielding base seat; inter-layer space between the internal stainless steel shielding layer, the secondary external stainless steel shielding layer and the external stainless steel shielding layer is filled with vacuum; a diameter ratio between the above three layers is determined via a heat loss equation. The heating device provided in the invention is high in heating efficiency, small in size and good in shielding effects; a problem that a conventional heating device affects normal work of the scanning electron microscope is overcome.