The invention relates to souffle muffin premixed flour and application thereof, and belongs to the field of baking food raw materials. The souffle muffin premixed flour is prepared from, by weight, 10-20 parts of low gluten wheat flour, 10-20 parts of corn starch, 5-15 parts of egg protein powder, 8-12 parts of polyglycerin fatty acid ester, 4-6 parts of double-effect baking powder, 0.3-0.5 part of acidity regulator, 1-3 parts of mono-diglycerin fatty acid ester, 1-2 parts of polyoxyethylene (20) sorbitan monooleate, 0.3-0.6 part of edible salt, 0.05-0.1 part of thickening stabilizer, 30-40 parts of white sugar and 3-8 parts of natural edible sweet sugar. souffle muffins prepared by using the souffle muffin premixed flour have the advantages that the batter mixing effect is good, the muffins are thick in appearance, low in hardness, softer in mouthfeel and plump and upright without half-baked taste or collapse.