5 results about "Melanocyte-stimulating hormone" patented technology

The application discloses a kind of serum-free epidermal melanocyte culture medium and its application, method includes by dissolving adenine, hepatocyte growth factor (HGF), glutamine, retinoic acid, vitamin E, nicotinic acid, sodium selenite, sodium hyaluronate and recombination human type XVII collagen are configured into initial culture medium;Preparation of melanocyte stimulating hormone, endothelin-3, transferrin, triiodothyronine, cholesterol, neuroregulin 1 (NRG1), 3-isobutyl-1-methyl purine (IBMX), dibutyryl cyclic adenosine monophosphate (db-cAMP), cholera toxin, hydrocortisone and recombination human insulin mother liquor;Step three: according to culture and concentration, a certain amount of mother liquor of step two is added to initial culture medium.The culture medium can effectively promote human epidermal melanocyte proliferation, melanin accumulation and help to promote human umbilical cord mesenchymal stem cells into human epidermal melanocyte, provide key advantage for obtaining functional melanocyte with high activity and therapeutic potential.

Provided is a use of α-MSH or a promoter thereof in liver cancer; specifically, provided is a use of α-MSH, an analog thereof or a promoter thereof for preparing a pharmaceutical composition or preparation for: (i) inhibiting the growth of liver cancer cells, and / or (ii) treating liver cancer. The pharmaceutical composition or preparation can induce apoptosis of liver cancer cells, reducing the number of liver cancer cells and thus achieving an anti-tumor effect.

The invention belongs to the technical field of biology, and particularly relates to a tea source peptide with whitening and anti-aging functions and a preparation method and application thereof. According to the invention, tea protein is subjected to alkaline protease enzymolysis to obtain tea protein hydrolysate (TPH), and the peptide fragment composition of the tea protein hydrolysate (TPH) is analyzed by using an ultra-high performance liquid chromatography-mass spectrometry (UPLC-MS / MS) technology to screen out the peptide fragment with melanin generation inhibition and anti-aging functions. Experimental results show that the tea source peptides EGFG and FGDPHG can effectively inhibit tyrosinase activity in a cell-free system and show a remarkable free radical scavenging effect. In melanoma cells, the two can significantly inhibit synthesis of melanin induced by alpha-melanotropin (alpha-MSH), and have a significant antioxidant effect in an aging model of H2O2 induced oxidative stress. The whitening and anti-aging effects are further proved in a zebra fish model. The tea source peptide provided by the invention has whitening and anti-aging effects, and can be used as an active component in whitening and anti-aging functional foods, cosmetics and medicines.

The present invention relates to alpha-melanocyte stimulating hormone analogs targeting the melanocortin-1 receptor.

The invention relates to application of alpha-MSH or an accelerant thereof in liver cancer, and particularly discloses application of alpha-MSH, an analogue thereof or an accelerant thereof and application of alpha-MSH, an analogue thereof or an accelerant thereof to preparation of a pharmaceutical composition or a preparation, and the pharmaceutical composition or the preparation is used for (i) inhibiting growth of liver cancer cells; and / or (ii) treating liver cancer. The pharmaceutical composition or the preparation can induce apoptosis of liver cancer cells and reduce the number of liver cancer cells, so that the anti-tumor effect is achieved.