67 results about "Neisseria sp" patented technology

The invention provides a method for detecting mutation of drug-resistant sites of 19 neisseria gonorrhoeae. The method utilizes a multiplex PCR-mass spectrometry method to detect the mutation of the drug-resistant sites of the 19 neisseria gonorrhoeae. The detection method comprises the following steps: 1) designing primers for different drug-resistant sites; 2) carrying out multiplex PCR amplification reaction; 3) treating shrimp alkaline phosphatase; 4) carrying out single base extension reaction; 5) desalting and purifying resin; and 6) carrying out mass spectrometric detection.

An aqueous immunogen formulation is used to reconstitute a lyophilised component including conjugates of capsular saccharides from Neisseria meningitidis serogroups A, C, W135 and Y, thereby producing a combined vaccine. The aqueous formulation can include various immunogens but does not include a Hib conjugate.

The invention belongs to the technical field of molecular biological detection, relates to a detection method for pathogenic microorganisms, and in particular relates to a visualization method for rapidly detecting neisseria gonorrhoeae by utilizing a recombinase polymerase isothermal amplification technology. According to the method, by combining the recombinase polymerase isothermal amplification technology and fluorescent dye developing, rapid amplification of neisseria gonorrhoeae nucleic acids can be realized under the condition that a PCR (Polymerase Chain Reaction) instrument and any other heat cycle equipment are not used. The results are identified by observing the color change of the reaction system with naked eyes. According to related reagents provided by the invention, the neisseria gonorrhoeae in samples can be rapidly detected by virtue of simple treatment of to-be-detected samples, and the detection method has the advantages of being simple in operation, high in specificity, capable of determining the results with naked eyes, and the like, and is applicable to clinical diagnosis and individual early screening.

Methods and compositions for immunizing a human subject against Neisseria gonorrhoeae.

Carrier-induced epitopic suppression is of particular concern where multiple conjugates with the same carrier protein are administered simultaneously. To avoid the suppression, the invention minimises the amount of unconjugated carrier protein in a vaccine. The invention provides a composition for immunising a patient against a disease caused by Neisseria meningitidis, wherein (1) the composition comprises conjugates for at least two of the four meningococcal serogroups A, C, W135 and Y, where at least two of the conjugates have a common carrier protein; and (2) the composition includes the common carrier in an unconjugated form at less than 10 μg/ml.

An 85kDa antigen from Neisseria meningitidis and Neisseria gonorrhoeae has been cloned, sequenced and expressed. The antigen is common to diverse strains, serogroups and serotypes of N. meningitidis, and also to N. gonorrhoeae, N. polysaccharia and N. lactamica. The protein sequences of N. meningitidis (serogroups A and B) and N. gonorrhoeae are highly homologous.

The invention provides a primer group for detecting specific typing sites of eight genital tract pathogenic bacteria and two human papilloma viruses by a time of flight mass spectrometry method, a product and a non-diagnostic detection method. The genital tract pathogenic bacteria comprise staphylococcus aureus, group B streptococcus, neisseria gonorrhoeae, gardnerella vaginalis, candida albicans, mycoplasma genitalium, mycoplasma urealyticum, chlamydia trachomatis, and HPV16 and HPV18 virus types. The invention provides a novel method for detecting 10 kinds of genital tract pathogens by combining multiple PCR with clinical mass spectrometry for the first time, integrates technologies of multiple PCR, single base extension, mass spectrometry detection and the like, can amplify a detection template through the PCR technology, can detect a trace sample through the mass spectrometry technology, integrates the advantages of the two technologies, is much superior to the method for detecting specific fragments of pathogens by singly using PCR, and has higher detection sensitivity.