46 results about "Plastid Genomes" patented technology

The instant disclosure describes the application of genetic engineering techniques to produce cellulase in plants. Cellulase coding sequences operably linked to promoters active in plants may be transformed into the nuclear genome and / or the plastid genome of a plant. As cellulases may be toxic to plants, chemically-inducible or wound-inducible promoters may be employed. Additionally, the expressed cellulases may be targeted to vacuoles or other cellular organelles.

The invention relates to a method for the production of plants with suppressed photo-respiration and improved CO2 fixation. In particular, the invention relates to a re-use of phosphoglycolate produced in photorespiration. The reaction product will be converted to a component that may be reintegrated into the plant assimilatory metabolism inside the chloroplast. This is accomplished by the transfer of genes derived from glycolate-utilizing pathways from bacteria, algae, plants and / or animals including humans into the plant nuclear and / or plastidial genome. The method of the invention leads to a reduction of photorespiration in C3 plants and by this will be of great benefit for food production especially but not exclusively under non-favourable growth conditions.

A plastid transformation vector for stably transforming a plastid genome, comprising, as operably-linked components, a first flanking sequence, at least one DNA sequence coding for a polypeptide suitable for remediating a contaminant compound, and a second flanking sequence, wherein a plant is stably transformed with the plastid transformation vector, and the plant is capable of phytoremediating a contaminant compound.

The invention relates to a method for detecting activity of a chloroplast promoter on the basis of a tetracycline regulation system, belonging to the technical field of plant plastogene engineering research. The method comprises the following steps: on the basis of the tetracycline gene and tetracycline specific recognition sequence core control region, biosynthesizing a prrnO1O2 promoter with the tetracycline core control region, verifying the activity of the promoter in prokaryotic cells, and carrying out GFP (green fluorescent protein) gene expression under the driving action of the promoter; establishing a tetracycline-induced expression vector, and screening out the tetracycline suitable for bacterium growth, of which the maximum concentration is 5 mu g / ml; and finally, establishing the GFP expression vector under the tetracycline regulation system, wherein the function of the prrnO1O2 promoter is inhibited when the tetracycline is not added, and the GFP gene expresses green fluorescent proteins after the tetracycline is added. The tetracycline regulation system is utilized to control the activity of the chloroplast promoter, thereby avoiding the regulation interference of the nuclear genome on the plastome, and providing an effective method and way for further plastogene engineering breeding.

The present invention is directed to Gene Positioning technology for biosynthesis of one or more products of interest via plastid transformation of plants or algae, such as for example tobacco, Lemna, Rhodomonas and Cryptomonas, with pseudogene vectors containing polynucleotides encoding one or more products of interest. The present invention is also directed to transgenic plants or algae, containing pseudogene vectors integrated into a desired locus in the plastid genome, allowing simultaneous expression of multiple transgenes.

A process for producing multicellular plants, plant organs or plant tissues transformed on their plastome by the following steps is provided: (a) altering or disrupting the function of a gene in a plastid genome for producing a selectable or recognizable phenotype; (b) separating or selecting plants or cells having plastids expressing said phenotype; (c) transforming said plastid genome of said separated or selected plant, plant organ or plant tissue with at least one transformation vector having a restoring sequence capable of restoring said function; and (d) separating or selecting said transformed plant, plant organ or plant tissue having plastids expressing said restored function.

This invention provides a novel method to confer disease resistance to plants. Plant plastids are transformed using a plastid vector which contains heterologous DNA sequences coding for a cytotoxic antimicrobial peptide. Transgenic plants are capable of fighting off phytopathogenic bacterial infection.

Disclosed is a method of making a malaria vaccine, the method comprising stably transforming a plant by inserting into its plastid genome a nucleic acid sequence encoding and operable to constitutively express a malaria antigenic polypeptide selected from AMA-1, MSP-1 or both; harvesting the stably transformed plant in whole or in part; purifying the expressed malaria antigenic polypeptide from the harvested plant; and packaging the purified antigenic polypeptide under sterile conditions in an amount for a predetermined dosage. Also disclosed is an oral vaccine effective in raising malaria antibodies in a susceptible host, the vaccine comprising leaf material from an edible plant containing plastids stably transformed to constitutively express a fusion polypeptide consisting essentially of cholera toxin B subunit and a malaria antigenic polypeptide selected from AMA-1, MSP-1 or both.

The invention discloses a construction method of porphyra yezoensis plastid genetic transformation vector, which comprises the steps of building skeleton vector containing rrsB-trnI-trnA-rrsL homologous recombination segments, building porphyra yezoensis plastid expression vector containing antibiotic screening gene expression cassettes, and building porphyra yezoensis plastid genome targeted integration expressed vector. The method adopts the thought of plastid genetic transformation, and provides a vector basis for plastid genetic transformation, so as to realize the advantages that the expression efficiency of plastid genetic transformation foreign gene is high, gene from pronucleus is not required to be modified, the safety is good, clon is easy to keep, and posterity is not separated.

This invention provides a method of conferring osmoprotection to plants. Plant plastid genomes, particularly the chloroplast genome, is transformed to express an osmoprotectant. The transgenic plants and their progeny display drought resistance. More importantly, such transgenic plants display no negative pleiotropic effects such as sterility or stunted growth.

Plants having transformed plastid genomes comprising a construct comprising attL and attR sites and a gene of interest, where the plants comprise an integrase and, optionally a selectable marker gene, and methods of making the plants.

A plastid transformation vector for a stably transforming a plastid genome is provided. The vector includes, as operably-linked components, a first flanking sequence, a DNA sequence coding for a therapeutic human IFN, which is capable of expression in the plastid and a second flanking sequence. The invention also provides isolated and purified IFN, wherein the IFN is configured in a monomeric or multimeric form and is a structural equivalent to orally administered human IFN. Also provided are methods for variable-expressing biopharmaceutical proteins in plants suitable for mammal consumption. The method includes integrating a plastid transformation vector into a plastid genome of a plant cell; growing the plant cell to express a biopharmaceutical protein, such as therapeutic human interferon IFN. Also disclosed are plants transformed with the aforementioned vectors, and the progeny thereof. Also, disclosed is the IFN, which is IFNα2b.

Disclosed is a method of making a malaria vaccine, the method comprising stably transforming a plant by inserting into its plastid genome a nucleic acid sequence encoding and operable to constitutively express a malaria antigenic polypeptide selected from AMA-1, MSP-1 or both; harvesting the stably transformed plant in whole or in part; purifying the expressed malaria antigenic polypeptide from the harvested plant; and packaging the purified antigenic polypeptide under sterile conditions in an amount for a predetermined dosage. Also disclosed is an oral vaccine effective in raising malaria antibodies in a susceptible host, the vaccine comprising leaf material from an edible plant containing plastids stably transformed to constitutively express a fusion polypeptide consisting essentially of cholera toxin B subunit and a malaria antigenic polypeptide selected from AMA-1, MSP-1 or both.

Compositions and methods for introducing heterologous sequences encoding a gene of interest into the plastid genome which do not rely on homologous recombination are disclosed.

The present invention relates to a method for the transformation of plastid genomes of plant species, in particular Asteraceae plant species, comprising the steps of providing a transformation vector carrying a DNA sequence of interest; subjecting a plant material, which comprises plastids, to a transformation treatment in order to allow the plastids to receive the transformation vector; placing the thus treated plant material for a period of time into contact with a culture medium without selection agent; subsequently placing the plant material into contact with a culture medium comprising a selection agent; and refreshing the culture medium comprising a selection agent to allow plant material comprising plastids that have acquired the DNA of interest to grow into transformants.

A method of producing plastid transformants including a process (a) in which genes coding for proteins having a function of neutralizing nitrite toxicity are introduced into plant tissues and a process (b) in which the plant tissues into which the genes are inserted in the process (a) are cultured in a medium containing nitrite nitrogen having a concentration at which growth of wild type plant cells is suppressed or a concentration at which regeneration of wild type plants is suppressed and thus plastid transformants obtained by inserting the genes into plastid genomes are screened.

Disclosed herein are methods of achieving male sterility in plants. Specifically exemplified herein is the transformation of the plastid genome with a vector expressing the phaA gene. Expression of the phaA gene in plastids results in plants that do not exhibit pleiotropic effects with the exception of male sterility. Also disclosed are stably transformed plants and cells, as well as example vectors for expressing the phaA gene in plastids.

Compositions and methods for manipulating the plastid genome of higher plants are provided.