4 results about "Plastid Genomes" patented technology

Compositions and methods for introducing targeted mutations into the plastid genome are provided.

The invention discloses molecular markers for identifying five caragana plants and application of the molecular markers, and relates to the technical field of molecular identification. The five caragana plants are caragana arborescens, caragana medii, caragana intermediary, rubus corchorifolius and caragana glandulifera; the molecular marker is shown as SEQ ID No.1, the molecular marker is located at the 4-582nd site of a caragana arborescens plastid genome, the total length of the molecular marker is 579 bp, and the insertion and deletion variation of the molecular marker occurs between the 196-198 site and the 442-443 site; the invention provides an important basis for the classification and identification of caragana plants, and lays a theoretical foundation for the subsequent development of adaptive evolution, germplasm resource protection and medicinal sustainable utilization research.

Provided are methods for plastid genome editing and development of plants, plant cells, plant parts, and seeds comprising edited plastid genomes. Compositions for transformation of plastid genomes are further provided. Specifically, the plastid genome is modified to comprise the replacement of a targeted endogenous plastid genome sequence with a modified version of the target plastid sequence, where the modified plastid genome sequence has been designed to deliberately reduce its homology to the targeted endogenous plastid genome sequence and, in some cases, encode a protein containing one or more mutations.

This invention provides a cytosine deaminase, a base editing system comprising the same, and their applications. The cytosine deaminase comprises an amino acid sequence having at least 80%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% sequence identity with the amino acid sequence shown in SEQ ID NO:1. The cytosine deaminase and two derived novel cytosine single-base editing tools of this invention are suitable for nuclear genome editing and mitochondrial plastid genome editing, solving problems such as narrow editing windows, transcriptome off-target effects, low editing efficiency, and 5'-TC sequence bias, thus expanding the selection and application range of gene editing and base editing tools. The mitochondrial cytosine base editor provided by this invention features small size, no sequence bias, and no restrictions, making it more suitable for viral vector-based gene therapy, and possessing good prospects for gene therapy and industrialization.