The invention provides a data acquisition designing method in three-dimensional seismic physical simulation, which mainly comprises a data acquisition design flow, a seismic physical model design technology and a three-dimensional transverse coverage frequency calculation formula and a three-dimensional observation system template decomposing and composing method in the three-dimensional seismic physical simulation experiment. The design technology of a key part in the design of a three-dimensional seismic physical model refers to the design technology of simplifying, laminarizing, exploration target outlining and abstracting a work-area subsurface geologic structure as well as the design technology of a seismic physical model scale factor. By applying the method, the work efficiency and the experiment achievement quality of a seismic physical model laboratory can be improved. In recent years, the laboratory cooperates with domestic Daqing, Jilin, Liaohe, Jidong, Dagang, Shengli, Xinjiang, Changqing, Renqiu, Zhongyuan, Jiangsu, Jianghan oil fields and the like to conduct scores of three-dimensional seismic physical model experiments, thereby obtaining better effect.