37 results about "Surface plasmon resonance biosensor" patented technology

An integrated optical waveguide based surface plasmon resonance biosensor is formed by detecting amplitude and phase of electromagnetic waves utilizing interferometry and / or optical delay configurations.

An objective of the present invention is to provide a measuring chip for a surface plasmon resonance sensor that can detect a small amount of target substances in high sensitivity. The present invention provides a measuring chip for a surface plasmon resonance sensor comprising a metal layer, one or more plasma polymerization layers formed on said metal layer, and a biologically active substance immobilized on the surface of said plasma polymerization layer.

A near-field surface plasmon detector is provided. The near-field surface plasmon detector includes one or more semiconductor layers that absorb one or more surface plasmons of thin metal films in the vicinity of the semiconductor layer. The surface plasmons are excited by incoming light being emitted from a light emitting source. The metal films are also employed as electrical contacts used to capture photocurrent generated after absorption of surface plasmons by the semiconductor layers.

An objective of the present invention is to provide a measuring chip for a surface plasmon resonance sensor that can detect a small amount of target substances in high sensitivity. The present invention provides a measuring chip for a surface plasmon resonance sensor comprising a metal layer, one or more plasma polymerization layers formed on said metal layer, and a biologically active substance immobilized on the surface of said plasma polymerization layer.

The present invention provides an SPR system and corresponding methods of use, for determining the presence or concentration of tumor-associated antigens in cancer patient samples. The SPR system may have multiple channels, with each channel having operably affixed thereto an antibody specific for a tumor-associated antigen, so as to allow detection of multiple tumor-associated antigens simultaneously. When a biological sample from a patient is applied to the SPR system, the presence of two or more tumor-associated antigens can be determined by measuring an SPR signal shift from each channel. The SPR system may detect the presence or concentration of a tumor-associated carbohydrate antigen, where the sensor surface contains affixed thereto an antibody specific for the glycosyl epitope, as well as an antibody specific for the polypeptide to which the carbohydrate antigen is naturally associated in cancer patients.

The present invention discloses a coupled waveguide-surface plasmon resonance biosensor, comprising: a grating layer formed of a transparent material, the grating layer comprising a first periodic grating structure; a waveguide layer formed on the first periodic grating structure, the refractive index of the waveguide layer being larger than the refractive index of the grating layer; a plasmon resonance layer formed on the waveguide layer, capable of being optically excited to cause a plasmon resonance wave; and a ligand layer formed on the plasmon resonance layer; capable of being bound to react with receptors of a sample to be tested.

The invention relates to a cell surface receptor biochip based on a surface plasmon resonance biosensor, a preparation method and application thereof. The biochip is characterized in that the chip is provided with a gold surface coating at a glass substrate and the gold surface is fixed with a sephadex layer which is fixed with the monoclonal antibody of the Beta subunit of the receptors of anti para-insulin and surface receptors are fixed by antibody capture. The preparation method includes that the monoclonal antibody of the Beta subunit of the receptors of anti para-insulin adopts the method of antibody capture and is fixed on the surface of CM5 chip based on the surface plasmon resonance biosensor, so as to produce the protein chip of para-insulin receptors which is applicable to the mutual action between IGF-1R and IRS-1, SHC, PI3K or GRB2 and hopeful to be applied to screening cancer-fighting drugs.

A surface plasmon resonance biosensor device and system are provided. The simplicity of SPR biosensor design allows easy integration with both QCM and electrochemistry techniques, not found in current SPR biosensor devices. In some embodiments, the surface plasmon resonance biosensor device has a dual SPR / QCM sample holder which allows simultaneous detection by both surface plasmon resonance and also quartz crystal microbalance (QCM) techniques. In additional embodiments, the surface plasmon resonance biosensor device and / or the dual SPR / QCM technique can be integrated with electrochemistry techniques by incorporate reference and counter electrodes in the SPR or SPR / QCM sample holder. Methods of using the device and system to determine whether an analyte of interest is present in a sample are also provided.

An assay apparatus of a surface plasmon resonance biosensor system includes a flow channel block loadable with a sensor unit of the chip type removably. A metal film has a sensing surface and a metal / dielectric interface being defined on a dielectric glass substrate. An optical assay unit applies illumination through the glass substrate to the metal / dielectric interface as conditioned for total reflection, detects the illumination reflected by the metal / dielectric interface during the interaction of the ligand and the analyte on the sensing surface. The assay apparatus includes a sensor storage for storing the sensor unit in an unused state. A used sensor receptacle receives the sensor unit in a used state after use in the assay. A transfer assembly retrieves the sensor unit for the assay from the sensor storage, sets the sensor unit on the flow channel block, and discharges the sensor unit toward the used sensor receptacle.

The invention relates to a prism-coupled SPR biosensor, which comprises an optical plastic prism, an inner metal film layer, an outer metal film layer and a biological component layer; The outer metal film layer is deposited on the film layer, the inner metal film layer and the outer metal film layer can be two kinds of the same or different metal film layers, and the sum of the thickness of the inner metal film layer and the outer metal film layer is between 20-80nm The space meets the optical requirements for exciting surface plasmon resonance, and a biological component layer is fixed on the surface of the outer metal film layer. The present invention also relates to the use of the prism-coupled SPR biosensor in preparing biochips and its use in detecting interactions between biological samples.

The invention relates to a periodic nanostructure-based biosensor and a preparation method thereof. The biosensor is a surface plasmon resonance biosensor, and comprises a substrate, a noble metal thin film and a periodic nanostructure, wherein the noble metal thin film is arranged on the substrate; the periodic nanostructure is arranged on the noble metal thin film; the periodic nanostructure comprises a plurality of through-hole structures which are equally distributed and are parallel to each other; and the periodic nanostructure is used for providing wave vector matching needed by exciting surface plasma. The periodic nanostructure-based biosensor can overcome disadvantages of huge size in a conventional biosensor and provide the wave vector matching needed by exciting the surface plasma. Besides, all production processes are compatible with those of a standard semiconductor technology. The periodic nanostructure-based biosensor is simple in production processes, has relatively small size and is easy to integrate.

An integrated optical waveguide based surface plasmon resonance biosensor is formed by detecting amplitude and phase of electromagnetic waves utilizing interferometry and / or optical delay configurations.

A method of generating surface plasmon resonance using excitation light directed at a thin metal film by a micromirror is described. A method that uses excitation light directed at a thin metal film by a micromirror scanner device is also described. A surface plasmon resonance imager is described comprising a micromirror that directs light to the surface of a thin metal film. Another method is described, comprising: a) directing light toward a thin metal film using a micromirror and b) detecting dynamic chemical events at or near the surface of the thin metal film. The dynamic events may be, for example, a fluidic change or a binding event.

An optical device having a structure which contains a metallic layer and enables excitation of plasmon resonance, such as a measurement chip for use with a surface-plasmon-resonance biosensor having a metallic layer. In order to prevent oxidation of the metallic layer, the metallic layer is covered with a film made of a mercapto compound containing a benzene ring and / or a hetero ring, or a reductive compound containing a metal-binding group which can be bound to the metal of which the metallic layer is made.

A near-field surface plasmon detector is provided. The near-field surface plasmon detector includes one or more semiconductor layers that absorb one or more surface plasmons of thin metal films in the vicinity of the semiconductor layer. The surface plasmons are excited by incoming light being emitted from a light emitting source. The metal films are also employed as electrical contacts used to capture photocurrent generated after absorption of surface plasmons by the semiconductor layers.

The present invention provides a method of detecting human antibodies in a sera solution. The invention also provides a method of quantitating anti-glycolipid antibody levels in solutions. The invention provides a method of diagnosing disease states, including neurological diseases, by quantitating a subject's antibody levels.

A spectroscopic technique for high-sensitivity, label free DNA quantification uses a shift in an optical resonance (whispering gallery mode, WGM) excited in a micron-sized optical cavity (e.g., a silica sphere) to detect and measure nucleic acids. The surface of the silica sphere is chemically modified with oligonucleotides. Hybridization to the target DNA leads to a red-shift of the optical resonance wavelength. The sensitivity of this resonance technique is higher than most optical single-pass devices such as surface plasmon resonance biosensors. Each microsphere can be identified by its unique resonance wavelength. Specific, multiplexed DNA detection may be provided by using two or more microspheres. The multiplexed signal from two or more microspheres illustrates that a single nucleotide mismatch in an 11-mer oligonucleotide can be discriminated with a high signal-to-noise of 54. This all-photonic WGM biosensor can be integrated on a chip, such as a semiconductor chip, which makes it an easy to manufacture, analytic component for a portable, robust lab-on-a-chip device.

The invention relates to a portable enhanced surface plasmon resonance biosensor and belongs to the field of heavy metals detection. The portable enhanced surface resonance biosensor as a GH-SPR (Goos-Hanchen surface plasmon resonance) sensor, comprising a waterproof module, wherein the waterproof module is connected with an external laser source through an adapter and is connected with an external control circuit through a connection port. ONP (open network provisioning) technology is combined with the GH-SPR sensor, and SPR coupling effect more significant than heavy metal ions themselves iscreated through connection or disconnection of DNA strands and additional AuNPs to the surface of the sensor. The sensor is simple in structure and easy to use; a DNA probe method has the advantagesof low sampling quantity, zero need for marking, isolation and purification, short detection time and the like; a DNA chip is reusable so that the cost is further reduced.

A surface plasmon resonance apparatus (10) includes a prism (12); a dielectric layer (90) contacting one face (16) of the prism (12); a conducting layer (14) having a first surface contacting the dielectric layer (90) and a second surface to contact a sample (22) to be analyzed by the surface plasmon resonance apparatus (10); and a light source (18) emitting an incident light beam (26) having a wavelength 0 entering the prism (12) and incident on the face (16) of the prism (12) contacted by the dielectric layer (90) at an adjustable incident angle alpha relative to a normal to the face (16) of the prism (12) contacted by the dielectric layer (90). The conducting layer (14) has a thickness that enables generation of slightly asymmetric coupled surface plasmons when the incident angle alpha is equal to a resonance angle determined by the wavelength 0 of the incident light beam (26), an index of refraction (n m ) of the conducting layer, and an index of refraction (n s ) of the sample (22).

The invention relates to a biological sensor based on periodical nano medium particles and a preparation method of the sensor. The biological sensor is a surface plasma resonance biological sensor and comprises a substrate, a noble metal thin film and a plurality of periodically arranged nano medium particles, wherein the noble metal thin film is arranged on the substrate; the plurality of periodically arranged nano medium particles are arranged on the noble metal thin film; and the plurality of nano medium particles are in bidimensional array-type periodical arrangement and are used for supplying wave vector matching required for exciting surface plasmas. By the biological sensor based on the periodical nano medium particles, the shortcoming of large volume of the conventional biological sensor is overcome; the wave vector matching required for exciting the surface plasmas can be provided; all manufacturing processes are compatible with a standard semiconductor process; the manufacturing processes are simple; the size is relatively small; and the biological sensor is easy to integrate.

The invention relates to a portable probing type surface plasma resonance biosensor and a preparation method and a detection method of the portable probing type surface plasma resonance biosensor. The sensor comprises strip optical glass, an inner meal reflecting film coats the bottom of the strip optical glass, a chromium film coats the side wall of the strip optical glass, a gold film coated with a biomolecular sensitive layer coats the nano chromium film, a collimator is arranged on an incident light path of the strip optical glass, and a polarizing film, an optical fiber collimator and an optical fiber spectrometer connected with the optical fiber collimator by a multimode optical fiber are sequentially arranged on an emergent light path. The inner meal reflecting film coats the bottom end of the strip optical glass, the chromium film and the gold film sequentially coat the side wall of the top part of the strip optical glass, the biomolecular sensitive layer is formed on the surface of the gold film, and BSA solution is used for sealing to obtain the probing type surface plasma resonance biosensor. The portable probing type surface plasma resonance biosensor is inserted into liquid in a test tube for SPR measurement, the system is simplified, and the portability of the sensor is achieved; and besides, the portable probing type surface plasma resonance biosensor overcomes the disadvantages of the existing probing type SPR sensor, the measurement mode is simple and the portability of the instrument is realized.

The present invention discloses a coupled waveguide-surface plasmon resonance biosensor, comprising: a grating layer formed of a transparent material, the grating layer comprising a first periodic grating structure; a waveguide layer formed on the first periodic grating structure, the refractive index of the waveguide layer being larger than the refractive index of the grating layer; a plasmon resonance layer formed on the waveguide layer, capable of being optically excited to cause a plasmon resonance wave; and a ligand layer formed on the plasmon resonance layer; capable of being bound to react with receptors of a sample to be tested.

An objective of the present invention is to provide a measuring chip for a surface plasmon resonance sensor that can detect a small amount of target substances in high sensitivity. The present invention provides a measuring chip for a surface plasmon resonance sensor comprising a metal layer, one or more plasma polymerization layers formed on said metal layer, and a biologically active substance immobilized on the surface of said plasma polymerization layer.

A method of coating an SPR biosensor specific for an analyte to reduce protein fouling, the method has the steps of providing an SPR biosensor, providing a solution of 11-mercaptoundecanol; incubating the SPR biosensor in the 11-mercaptoundecanol solution to form a self-assembling monolayer (SAM); incubating the SPR with SAM in a solution of epichlorohydrin and diglyme; next incubating the SPR in ethanolamine; preparing a solution of EDCNHS and a biocompatible polymer; incubating the SPR from ethanolamine in the EDC / NHS / polymer solution; providing a ligand specific for the analyte in a solution; incubating the polymer-coated SPR in the ligand solution to permit the ligand to react with the polymer-coated SPR; washing the ligand-coated SPR to remove unreacted ligand, thereby providing an SPR capable of reacting with the analyte. Another method replaces the solution for the SAM layer with a solution of MHA or NHS-MHA with HT, and attaches the ligand to the resulting SAM layer.

The invention provides a detection method of an end face integrated reflection type SPR optical fiber biosensor manufactured based on a 2PP technology. An experiment system mainly comprises a single-mode optical fiber, an optical fiber coupler, an SPR probe, a tungsten lamp light source, a sample pool, a spectrum analyzer and a computer. The method is characterized in that a volcanic three-dimensional microstructure is directly constructed on the end face of a single-mode fiber through a 2PP technology, a metal film with the thickness of about 50nm is deposited on the inner wall of the volcanic three-dimensional microstructure, and a stable and efficient SPR excitation structure is formed; the structural parameters are subjected to FDTD simulation optimization design, and have the characteristics of high quality factor and high sensitivity. The sensor can be used for AFP detection, the sensitivity reaches 0.684 nm / (ng / mL) within the linear range of 0-10 ng / mL, the normal and risk early warning concentration interval of human serum AFP is covered, and the sensor has good biological suitability and early hepatocellular carcinoma screening capacity; in addition, the probe structure is small in size, detection can be completed only by a few microliters of liquid to be detected, and the probe is suitable for a microfluidic platform with an extremely low sample size or a clinical sampling scene. Compared with the prior art, the device has the advantages of high structural integration, high sensitivity, low sample consumption, convenience in manufacturing, stability, reliability and the like.

A surface plasmon resonance biosensor device and system are provided. The simplicity of SPR biosensor design allows easy integration with both QCM and electrochemistry techniques, not found in current SPR biosensor devices. In some embodiments, the surface plasmon resonance biosensor device has a dual SPR / QCM sample holder which allows simultaneous detection by both surface plasmon resonance and also quartz crystal microbalance (QCM) techniques. In additional embodiments, the surface plasmon resonance biosensor device and / or the dual SPR / QCM technique can be integrated with electrochemistry techniques by incorporate reference and counter electrodes in the SPR or SPR / QCM sample holder. Methods of using the device and system to determine whether an analyte of interest is present in a sample are also provided.

The invention provides an interlayer surface plasmon resonance biosensor which comprises a patterned substrate, and detection areas which are arranged in an array and are formed by metal layers are formed on the surface of the patterned substrate; the spacing layer is formed in the detection area and comprises capture molecules; the nanometer adhesive tape is compounded on the surface of the patterned substrate, and a metal nanoparticle layer is arranged on one surface, facing the patterned substrate, of the nanometer adhesive tape. The invention further provides a preparation method and application of the interlayer surface plasma resonance biosensor. The nano double-sided adhesive tape is used as a transfer medium, and stable transfer of the metal nanoparticles from the substrate to the adhesive tape can be realized through two-step operation of'attaching-stripping ', so that the transfer difficulty of the metal nanoparticle layer is remarkably reduced, the operation is simplified, and the transfer efficiency is improved. And parameters such as the size, the morphology and the density of the nanoparticles in the sandwich structure can be accurately controlled, and the obtained metal nanoparticle layer is good in stability and can be stored for a long time.

A surface plasmon resonance biosensor uses coupled surface plasmons to decrease the width of a reflectivity dip and thereby increase the sensitivity of the surface plasmon resonance biosensor.

The invention discloses a multichannel parallel detection surface plasma resonance (SPR) biosensor, and preparation and detection methods thereof. A sensing unit of the sensor comprises a triangular prism and a gold thin film lattice chip prepared on an optical slide, and cross-linked molecular layers and bioprobe molecules are assembled on the surfaces of lattice points; a halogen tungsten lamp, a precision monochrometer, a spherical lens, a diaphragm, a plano-convex lens and a polarizing film are arranged on an incident light path of the sensor in sequence; a convex lens and a CCD (charge coupled device) video camera are arranged on an emergent light path of the sensor in sequence, and the CCD video camera and the precision monochrometer are connected with a computer. According to a system, the SPR spectrum resonance curves of the lattice points are obtained in parallel by an optical sweeping mode, different biological targets are detected in parallel according to the motion of the SPR peaks of the lattice points, and the sensor has single-channel SPR sensitivity while being used for multichannel parallel detection.