35 results about "Surface plasmon resonance biosensor" patented technology

An integrated optical waveguide based surface plasmon resonance biosensor is formed by detecting amplitude and phase of electromagnetic waves utilizing interferometry and/or optical delay configurations.

An objective of the present invention is to provide a measuring chip for a surface plasmon resonance sensor that can detect a small amount of target substances in high sensitivity. The present invention provides a measuring chip for a surface plasmon resonance sensor comprising a metal layer, one or more plasma polymerization layers formed on said metal layer, and a biologically active substance immobilized on the surface of said plasma polymerization layer.

The present invention provides an SPR system and corresponding methods of use, for determining the presence or concentration of tumor-associated antigens in cancer patient samples. The SPR system may have multiple channels, with each channel having operably affixed thereto an antibody specific for a tumor-associated antigen, so as to allow detection of multiple tumor-associated antigens simultaneously. When a biological sample from a patient is applied to the SPR system, the presence of two or more tumor-associated antigens can be determined by measuring an SPR signal shift from each channel. The SPR system may detect the presence or concentration of a tumor-associated carbohydrate antigen, where the sensor surface contains affixed thereto an antibody specific for the glycosyl epitope, as well as an antibody specific for the polypeptide to which the carbohydrate antigen is naturally associated in cancer patients.

The present invention discloses a coupled waveguide-surface plasmon resonance biosensor, comprising: a grating layer formed of a transparent material, the grating layer comprising a first periodic grating structure; a waveguide layer formed on the first periodic grating structure, the refractive index of the waveguide layer being larger than the refractive index of the grating layer; a plasmon resonance layer formed on the waveguide layer, capable of being optically excited to cause a plasmon resonance wave; and a ligand layer formed on the plasmon resonance layer; capable of being bound to react with receptors of a sample to be tested.

The invention relates to a cell surface receptor biochip based on a surface plasmon resonance biosensor, a preparation method and application thereof. The biochip is characterized in that the chip is provided with a gold surface coating at a glass substrate and the gold surface is fixed with a sephadex layer which is fixed with the monoclonal antibody of the Beta subunit of the receptors of anti para-insulin and surface receptors are fixed by antibody capture. The preparation method includes that the monoclonal antibody of the Beta subunit of the receptors of anti para-insulin adopts the method of antibody capture and is fixed on the surface of CM5 chip based on the surface plasmon resonance biosensor, so as to produce the protein chip of para-insulin receptors which is applicable to the mutual action between IGF-1R and IRS-1, SHC, PI3K or GRB2 and hopeful to be applied to screening cancer-fighting drugs.

A surface plasmon resonance biosensor device and system are provided. The simplicity of SPR biosensor design allows easy integration with both QCM and electrochemistry techniques, not found in current SPR biosensor devices. In some embodiments, the surface plasmon resonance biosensor device has a dual SPR/QCM sample holder which allows simultaneous detection by both surface plasmon resonance and also quartz crystal microbalance (QCM) techniques. In additional embodiments, the surface plasmon resonance biosensor device and/or the dual SPR/QCM technique can be integrated with electrochemistry techniques by incorporate reference and counter electrodes in the SPR or SPR/QCM sample holder. Methods of using the device and system to determine whether an analyte of interest is present in a sample are also provided.

The invention relates to a periodic nanostructure-based biosensor and a preparation method thereof. The biosensor is a surface plasmon resonance biosensor, and comprises a substrate, a noble metal thin film and a periodic nanostructure, wherein the noble metal thin film is arranged on the substrate; the periodic nanostructure is arranged on the noble metal thin film; the periodic nanostructure comprises a plurality of through-hole structures which are equally distributed and are parallel to each other; and the periodic nanostructure is used for providing wave vector matching needed by exciting surface plasma. The periodic nanostructure-based biosensor can overcome disadvantages of huge size in a conventional biosensor and provide the wave vector matching needed by exciting the surface plasma. Besides, all production processes are compatible with those of a standard semiconductor technology. The periodic nanostructure-based biosensor is simple in production processes, has relatively small size and is easy to integrate.

A method of generating surface plasmon resonance using excitation light directed at a thin metal film by a micromirror is described. A method that uses excitation light directed at a thin metal film by a micromirror scanner device is also described. A surface plasmon resonance imager is described comprising a micromirror that directs light to the surface of a thin metal film. Another method is described, comprising: a) directing light toward a thin metal film using a micromirror and b) detecting dynamic chemical events at or near the surface of the thin metal film. The dynamic events may be, for example, a fluidic change or a binding event.

An optical device having a structure which contains a metallic layer and enables excitation of plasmon resonance, such as a measurement chip for use with a surface-plasmon-resonance biosensor having a metallic layer. In order to prevent oxidation of the metallic layer, the metallic layer is covered with a film made of a mercapto compound containing a benzene ring and/or a hetero ring, or a reductive compound containing a metal-binding group which can be bound to the metal of which the metallic layer is made.

A near-field surface plasmon detector is provided. The near-field surface plasmon detector includes one or more semiconductor layers that absorb one or more surface plasmons of thin metal films in the vicinity of the semiconductor layer. The surface plasmons are excited by incoming light being emitted from a light emitting source. The metal films are also employed as electrical contacts used to capture photocurrent generated after absorption of surface plasmons by the semiconductor layers.

A spectroscopic technique for high-sensitivity, label free DNA quantification uses a shift in an optical resonance (whispering gallery mode, WGM) excited in a micron-sized optical cavity (e.g., a silica sphere) to detect and measure nucleic acids. The surface of the silica sphere is chemically modified with oligonucleotides. Hybridization to the target DNA leads to a red-shift of the optical resonance wavelength. The sensitivity of this resonance technique is higher than most optical single-pass devices such as surface plasmon resonance biosensors. Each microsphere can be identified by its unique resonance wavelength. Specific, multiplexed DNA detection may be provided by using two or more microspheres. The multiplexed signal from two or more microspheres illustrates that a single nucleotide mismatch in an 11-mer oligonucleotide can be discriminated with a high signal-to-noise of 54. This all-photonic WGM biosensor can be integrated on a chip, such as a semiconductor chip, which makes it an easy to manufacture, analytic component for a portable, robust lab-on-a-chip device.

There is provided a method of detecting a pathogenic microorganism in real-time, using a modified flow-type surface plasmon resonance (SPR) biosensor, comprising the steps of: i) performing, in a batch-type, an immune reaction of a pathogenic microorganism and an antibody thereto; ii) selectively separating the pathogenic microorganism bound with the antibody; and iii) binding the pathogenic microorganism bound with the antibody on a surface of a chip of a flow-type SPR sensor system in real-time.

The invention relates to a biological sensor based on periodical nano medium particles and a preparation method of the sensor. The biological sensor is a surface plasma resonance biological sensor and comprises a substrate, a noble metal thin film and a plurality of periodically arranged nano medium particles, wherein the noble metal thin film is arranged on the substrate; the plurality of periodically arranged nano medium particles are arranged on the noble metal thin film; and the plurality of nano medium particles are in bidimensional array-type periodical arrangement and are used for supplying wave vector matching required for exciting surface plasmas. By the biological sensor based on the periodical nano medium particles, the shortcoming of large volume of the conventional biological sensor is overcome; the wave vector matching required for exciting the surface plasmas can be provided; all manufacturing processes are compatible with a standard semiconductor process; the manufacturing processes are simple; the size is relatively small; and the biological sensor is easy to integrate.

The invention relates to a portable probing type surface plasma resonance biosensor and a preparation method and a detection method of the portable probing type surface plasma resonance biosensor. The sensor comprises strip optical glass, an inner meal reflecting film coats the bottom of the strip optical glass, a chromium film coats the side wall of the strip optical glass, a gold film coated with a biomolecular sensitive layer coats the nano chromium film, a collimator is arranged on an incident light path of the strip optical glass, and a polarizing film, an optical fiber collimator and an optical fiber spectrometer connected with the optical fiber collimator by a multimode optical fiber are sequentially arranged on an emergent light path. The inner meal reflecting film coats the bottom end of the strip optical glass, the chromium film and the gold film sequentially coat the side wall of the top part of the strip optical glass, the biomolecular sensitive layer is formed on the surface of the gold film, and BSA solution is used for sealing to obtain the probing type surface plasma resonance biosensor. The portable probing type surface plasma resonance biosensor is inserted into liquid in a test tube for SPR measurement, the system is simplified, and the portability of the sensor is achieved; and besides, the portable probing type surface plasma resonance biosensor overcomes the disadvantages of the existing probing type SPR sensor, the measurement mode is simple and the portability of the instrument is realized.

A surface plasmon resonance biosensor uses coupled surface plasmons to decrease the width of a reflectivity dip and thereby increase the sensitivity of the surface plasmon resonance biosensor.