The invention belongs to the technical field of toothpaste, and particularly relates to toothpaste containing bioactive glass components and a preparation method of the toothpaste. The toothpaste comprises the following raw materials: bioactive glass, an abrasive, a solubilizer, a moisturizer, a surfactant, a sweetening agent and a preservative. By using the bioactive glass, the toothpaste not only has the traditional effects of resisting allergy, preventing tooth decay, strengthening and whitening teeth, stopping bleeding, diminishing inflammation, removing peculiar smell and the like, but also has the effects of repairing shallow pits and solving the problem of dental ulcer, the abrasive in the toothpaste can act together with a toothbrush to wipe tartar on the surfaces of the teeth andreduce solid raw material components of foreign substances such as tooth stains, dental plaque, dental calculus and the like, and a thickening agent can enable the toothpaste to have proper viscosityand consistence and enable the toothpaste not to be sticky or greasy; the toothpaste is provided with a framework, so that the extruded toothpaste can stay on the toothbrush without collapsing; duringtooth brushing, the toothpaste is easy to disperse and has good diffusion performance; and moreover, the paste is fine and glossy, and the abrasive is uniformly dispersed and does not get sticky or become coarse.