The invention provides a mouse control method and application thereof, and the method comprises the steps: building a selection table P of an initial DPI value of a mouse, recognizing the type of a real-time program D0, responding to the mouse in real time, on a host, selecting a DPI value matched with the type of the program from the selection table P, and setting the DPI value as the initial DPI value of the mouse. According to the method, the mouse DPI values can be primarily selected according to the program types, the selection efficiency is high, the calculation amount can be remarkably reduced, and the primarily selected mouse DPI values which are relatively adaptive can be provided for different types of programs. After primary selection, the mouse DPI value is adjusted for the first time and more than one time of subsequent adjustment to complete adjustment of the mouse DPI value, so that the mouse DPI value can be further matched with a corresponding program type, and compared with the method of selecting the DPI value by adopting an optimal iteration algorithm and the like in the prior art, the calculation amount can be remarkably reduced, the workload of a host is reduced, and crash of the host or occupation of a large number of computing resources is avoided, and the adjustment duration is short.