The invention relates to a multifunctional external sealing device for a medical operation puncturing device. The multifunctional external sealing device for the medical operation puncturing device comprises an upper pressing cover, a lower pressing cover, a base, a supporting base, a top base, a sealing film assembly, a supporting piece assembly and a sealing cushion assembly, wherein the upper pressing cover is fixed with the lower pressing cover, the sealing film assembly is installed between the base and the supporting base, the supporting piece assembly is installed between the supporting base and the sealing cushion assembly, the sealing cushion assembly is installed between the supporting piece assembly and the top base, the base, the supporting base, the top base, the sealing film assembly, the supporting piece assembly and the sealing cushion assembly are installed in the upper pressing cover and the lower pressing cover, and the sealing cushion assembly and the lower pressing cover are fixed. The multifunctional external sealing device for the medical operation puncturing device is reasonable in design, good in sealing effect, long in service life, and suitable for instruments with different external diameters and specifications, ensures that an operation is more conveniently and simply and faster conducted, and effectively shortens operation time.