The invention provides a working method of an assembling device for a medical one-off 
oxygen humidification 
bottle. The assembling device comprises a humidifying plug charging device, a transferring device, a ventilating 
pipe charging device, a ventilating 
pipe assembling device, a first assembling device, a second assembling device and a third assembling device, wherein the ventilating 
pipe charging device comprises a material box, a first 
conveyor belt, a charging block and an aligning 
assembly; the bottom part of the material box is obliquely arranged; a plurality of charging strips are arranged on the first 
conveyor belt; charging grooves are obliquely formed in the middle part of the charging block; the aligning 
assembly comprises an aligning motor, an aligning block and four aligningstrips; the cross section of the aligning block is of a rectangular structure, and four aligning grooves which are circumferentially distributed are formed in the aligning block; and the four aligning strips are fixedly connected to the aligning block. The method has the beneficial effects that a humidifying plug, a ventilating pipe, a connecting plug, a 
bottle body and a 
bottle cap can be automatically assembled, so that the product production cost is decreased; and the difficulty in production of the one-off 
oxygen humidification bottle can be increased.