The invention discloses a near-infrared fluorescence spectrophotometer based on a miniature scanning grating micromirror. The near-infrared fluorescence spectrophotometer comprises a detection chamber, a fluorescence excitation module and a near-infrared fluorescence splitting and spectrum obtaining module, rapid acquisition of near-infrared full-spectrum information is realized by integrating the scanning grating micromirror, a three-dimensional full spectrum of a measured substance can be obtained within one minute by combining with the fluorescence excitation module, and the influence of coma can be effectively reduced by the fluorescence excitation module; a plano-convex cylindrical mirror in the near-infrared fluorescence splitting and spectrum acquisition module is utilized to ensure the imaging quality of near-infrared fluorescence spectrum, aberration caused by an optical system in the fluorescence spectrum information acquisition process is greatly reduced, the spectral resolution and the signal-to-noise ratio are improved, near-infrared spectrum information detection is realized by utilizing a single-tube detector, the spectrum information acquisition speed is ensured, the structure of an optical system is simplified, the cost is reduced, and the system has the advantages of modularization, miniaturization, wide spectrum, high resolution, high signal-to-noise ratio, low cost, high detection speed and the like.