The invention provides an airtightness detection device for a seal, comprising: a seal unit, including a mounting table and a pressing mechanism, the mounting table is provided with a sealing head, and when the sealing member is located on the mounting table, the sealing head can be inserted into the opening of the seal and seal the opening, the pressing mechanism is used to abut against the seal and press the part around the opening of the seal on the plugging head; the air supply unit is provided with an air supply pipe, And when the gas supply unit is activated, it can output gas along the gas supply pipe, wherein the gas supply pipe is used to connect with the mounting platform or the sealing part, and when the sealing unit and the sealing part are assembled, it forms a closed cavity with the sealing unit and the sealing part. The detection device provided by this scheme can form a tightly sealed airtight cavity when the seal is assembled with it, and air can be fed into the airtight cavity to realize the detection of trachoma on the seal by using the air tightness detection method, false detection and missed detection The risk is small, and the loading and unloading operation between the sealing unit and the sealing member is also very simple, and the detection cost is low.