The invention discloses an optical fingerprint acquisition device for preventing interference of covering, and relates to the field of optical fingerprint acquisition, the optical fingerprint acquisition device comprises a main body shell, one side of the main body shell is provided with an inclined plane shell, the end face of the inclined plane shell is provided with a frame, the frame is internally provided with glass, and the bottom end of the interior of the inclined plane shell is provided with a light emitting diode; a charge coupler is installed on the inner wall of the inclined face shell, a single chip microcomputer is installed on the end face of the main body shell, a clamping groove is formed between the main body shell and the inclined face shell, the clamping groove is in an L shape, and outer edge shells are obliquely arranged on the two sides of the inclined face shell. According to the device, through arrangement of a pulling plate, bristles can be pushed to move to the outer side of a protective film, cleaning liquid obtained after cleaning can be collected, waste cleaning liquid is prevented from flowing randomly, unnecessary damage to the device is avoided, operation of a pushing motor is controlled through extrusion of fingers on glass, and the cleaning efficiency is improved.