The invention relates to a medicine patch for conditioning eye refraction and a preparation method of the medicine patch. The medicine patch comprises a laminating carrier attached with a medicine, wherein the medicine is prepared from the following raw materials in parts by weight: 4-8 parts of muscone, 4-8 parts of pearl powder, 8-12 parts of cassia occidentalis, 4-8 parts of semen plantaginis, 6-10 parts of goldthread root, 6-10 parts of salviae miltiorrhizae, 4-8 parts of borneol, 6-10 parts of golden cypress, 6-10 parts of buddleja officinalis and 2-3 parts of ginger oil. The medicine patch for conditioning eye refraction disclosed by the invention is capable of clearing heat, decreasing internal heat, reducing swelling, moistening eyes and enhancing cycle, and has a good conditioning treatment effect on myopia, amblyopia and astigmatism caused by ametropia.