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33 results about "Hepatitis C virus RNA" patented technology

Inhibitors of hepatitis C virus RNA-dependent RNA polymerase, and compositions and treatments using the same

The present invention provides compounds of formula (4), and their pharmaceutically acceptable salts and solvates, which are useful as inhibitors of the Hepatitis C virus (HCV) polymerase enzyme and are also useful for the treatment of HCV infections in HCV-infected mammals. The present invention also provides pharmaceutical compositions comprising compounds of formula (4), their pharmaceutically acceptable salts and solvates. Furthermore, the present invention provides intermediate compounds and methods useful in the preparation of compounds of formula (4).
Owner:AGOURON PHARMA INC +1

Pseudovirion containing hepatitis c virus RNA (Ribonucleic Acid) fragment and preparation method thereof

The invention provides a pseudovirion containing hepatitis c virus (HCV) RNA (Ribonucleic Acid) fragment and preparation method thereof which are applied in the field of biomedical clinical vitro diagnostic. The pseudovirion is a RNA-protein complexes that MS2 bacteriophage capsid protein wraps the hepatitis c virus, and the complexes is icosahedral; the method comprises the following steps of designing and synthesizing primer to obtain target gene MS2 by overlapping and splicing PCR (Polymerase Chain Reaction) method; connecting the target gene MS2 to plasmid pET-32a (+) to obtain recombinant plasmid pET-MS2; connecting the recombinant plasmid pET-MS2 and the HCV fragment to obtain pET-MS2-HCV recombinant plasmid; the obtained pET-MS2-HCV recombinant plasmid is introduced into escherichia coli for prokaryotic expression; releasing virus-like particles by adopting ultrasonication and the obtained virus-like particles are the pseudovirion containing the hepatitis c virus RNA fragment. The pseudovirion preparation method provided by the invention has the advantages that the preparation method is simple, the operation is easy, the obtained pseudovirus is high in purity, and the pseudovirion can be used as standard and quality control material of detection of RT-PCR (Reverse Transcription-Polymerase Chain Reaction), is good in stability, has no infectivity, has RNase-resistant, and the like.
Owner:宝瑞源生物技术(北京)有限公司

In vitro activity measuring method for hepatitis C virus RNA depending RNA polymerase and application thereof

The invention provides a detecting method and an application of the extraneous activity of the RNA polymerase relied on by the hepatitis C virus RNA, which relates to an secure and practical establishing method for the detection of the activity extraneous detection of the RNA polymerase relied on by the hepatitis C virus RNA, and the application relates to the genetic engineering technology and the nanometer segregation enrichment and RNA related technology field. The invention expresses the soluble NS5B protein through the utilization of the genetic engineering technology, and prepares the nanometer magnetic particle. One end of a RNA template is fixed on the nanometer magnetic particle, and added in the 96 hole reaction plate micromesh, and added with NS5B protein to compose a RNA duplication system, and a duplicated RNA double chain on the nanometer magnetic particle is mixed with marked biological elements. After the RNA is duplicated, the nanometer magnetic particle is attached at the micromesh bottom through an external magnetic field magnet, after repeatedly cleaning and attaching, the nanometer magnetic particle is combined with the marked combining factor of the horse radish peroxidase, after attaching and cleaning, the substrate constant of the horse radish peroxidase is added to develop color. New NS5B protease activity inhibitor can be screened from a traditional Chinese storeroom and a compound storeroom by the established method.
Owner:李越希

Hepatitis C sequencing and typing kit and detection method thereof

The invention discloses a hepatitis C sequencing and typing kit which comprises: (1) primers of specific amplified hepatitis C virus RNA reverse transcription product c DNA and (2) hepatitis C virus specific sequencing primers, wherein the nucleotide sequences of the primers of specific amplified hepatitis C virus RNA reverse transcription product c DNA are shown as SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3 and SEQ ID NO:4, the SEQ ID NO:1 and the SEQ ID NO:2 are a primer pair and the SEQ ID NO:3 and the SEQ ID NO:4 are another primer pair; and the nucleotide sequences of the hepatitis C virus specific sequencing primers are shown as SEQ ID NO:5, SEQ ID NO:6 and SEQ ID NO:7; the SEQ ID NO:4 is marked with a biotin at a 5' end; and all the primers are all used in a detection. The invention also discloses a detection method of the hepatitis C sequencing and typing kit. The hepatitis C sequencing and typing kit can be used for sequencing and typing hepatitis C, thereby providing more comprehensive reference information for clinical diagnosis and treatment.
Owner:CHONGQING DIAN SRAB CENT FOR CLINICAL LAB CO LTD

Isothermal chain multiple detection card of pathogen nucleic acid

The invention discloses an isothermal chain multiple displacement detection card of pathogen nucleic acid. The isothermal chain multiple displacement detection card of the pathogen nucleic acid utilizes the detection card which is prepared through a nucleic acid isothermal chain displacement method and a colloidal gold detection technology to carry out multiple detection on HBV-DNA, HCV-RNA, HIV-RNA, and TP-DNA. The isothermal chain multiple displacement detection card of the pathogen nucleic acid has the characteristics of simple and rapid operation, high sensitivity, and no need of professional equipment. The pathogen nucleic acid to be detected is not amplified in the detection process, so the detection card has the advantages of preventing amplified matter cross contamination in laboratories and preventing false positivity, can be widely used in high sensitivity pathogen nucleic acid detection in the fields of clinical detection, inspection and quarantine, infectious disease control, biological technology and the like, and has a wide application prospect.
Owner:武汉中科志康生物科技有限公司

Multi-color fluorescent PCR detection kit for nucleic acid testing and genetic typing of hepatitis c virus and application

The invention discloses a multi-color fluorescent PCR kit for nucleic acid testing and genetic typing of hepatitis c virus and an application thereof. The kit comprises RT-PCR reaction liquid, enzyme mixed liquid, HCV reaction liquid I, HCV reaction liquid II and HCV reaction liquid III, and the kit preferably also comprises HCV typing negative control, HCV typing positive control and application instruction. For the extracted HCV RNA, the hepatitis c virus RNA and the subtype of the hepatitis c virus in a test sample can be rapidly detected by adopting three-tube multi-color fluorescent PCR through one-step RT-PCR. The amplification method of the kit is simple, simplicity in operation is realized, the testing result is high in sensitivity, the specificity is good, the kit can be used for testing and genetic typing of HCV RNA in human serum or plasma, the judgment on the treatment difficulty can be favored, the establishment of an individual antivirus treatment scheme is favored, and the kit has the clinical popularization value.
Owner:JIANGSU BIOPERFECTUS TECH CO LTD

Method for HCV RNA real-time fluorescent detection with two probes

The disclosed dual-probe real-time detection method for hepatitis-C virus RNA comprises: preparing the primer and probe, preparing the reaction solution and RNA sample, and quantitative detecting. This invention improves the sensitivity and specificity for HCVRNA detection.
Owner:AFFILIATED HOSPITAL OF NANTONG UNIV

Direct detection of unamplified hepatitis c virus RNA using unmodified gold nanoparticles

InactiveUS20130236880A1Simple, rapid, and sensitive colorimetric assaySensitively detect HCVMicrobiological testing/measurementNanosensorsNanoparticleHepatitis C virus RNA
A gold nanoparticle-based colorimetric assay kit for hepatitis C virus RNA that detects unamplified HCV RNA in clinical specimens using unmodified AuNPs and oligotargeter polynucleotides that bind to HCV RNA. A method for detecting hepatitis C virus comprising contacting a sample suspected of containing hepatitis C virus with a polynucleotide that binds to hepatitis C virus RNA and with gold nanoparticles, detecting the aggregation of nanoparticles, and detecting hepatitis C virus in the sample when the nanoparticles aggregate (solution color becomes blue) in comparison with a control or a negative sample not containing the virus when nanoparticles do not aggregate (solution color remains red).
Owner:THE AMERICAN UNIV OF CAIRO

Novel inhibitors of hepatitis C virus

The invention provides compounds of formula (I) wherein the variables are defined in the specification, or a pharmaceutically-acceptable salt thereof, that are inhibitors of replication of the hepatitis C virus. The invention also provides pharmaceutical compositions comprising such compounds, methods of using such compounds to treat hepatitis C viral infections, and processes and intermediates useful for preparing such compounds.
Owner:THERAVANCE BIOPHARMA R&D IP LLC

Hepatitis C virus inhibitors

The present disclosure relates to compounds, compositions and methods for the treatment of hepatitis C virus (HCV) infection. Also disclosed are pharmaceutical compositions containing such compounds and methods for using these compounds in the treatment of HCV infection.
Owner:BRISTOL MYERS SQUIBB CO

Truncated type hepatitis c virus HCV NS3 antigen and preparation method and application thereof

ActiveCN105254724AEfficient developmentHigh positive serum coverageBacteriaVirus peptidesAntigenEscherichia coli
The invention discloses a truncated type hepatitis c virus HCV NS3 antigen which is a nonstructural protein amino acid sequence of the NS3 full-length gene encoded 1252th-1526aath segment in an HCV genome. The amino acid sequence is shown as SEQ ID NO.1, and a corresponding nucleotide sequence is shown as SEQ ID NO.2. A preparation method of HCV NS3 recombinant antigen protein comprises the steps that an optimized escherichia coli codon for expressing the truncated type hepatitis c virus HCV NS3 antigen are established, the nucleotide sequence of the codon is shown as SEQ ID NO.3, the gene segment of the codon undergoes double enzyme digestion and then is connected with an expression vector undergoing the enzyme digestion as well to obtain a recombinant expression plasmid, the antigen can perform expression by adopting a conventional method, and the antigen protein is obtained through purification. The antigen protein has high sensitivity and good specificity and is used for detecting an HCV antibody, and the coincidence rate of a detection result of a patient with positive hepatitis c virus RNA is as high as 97%. It is indicated that the antigen can be applied to polyclonal or monoclonal antibodies and preparation of hepatitis c virus detection kits and has good prospect of clinical application.
Owner:山东莱博生物科技有限公司

Hepatitis C virus HVR1 (Hypervariable Region 1) fusion antigen and application thereof

The invention discloses a hepatitis C virus HVR1 (Hypervariable Region 1) fusion antigen. An amino acid sequence of the fusion antigen is shown as SEQ ID No.1. The invention also discloses application of the fusion antigen in preparation of a hepatitis C virus HVR1 antibody detection kit. The experiment proves that the cross reactivity of the fusion antigen is obviously improved compared with that of a single segment HVR1 antigen, and the detection coincidence rate of hepatitis C virus RNA positive patients is 99 percent. Because the HVR1 is a main neutralizing antigen epitope in the hepatitis C virus, the antigen can be used for achieving an aim of comprehensively detecting HCV infection for hepatitis C virus HVR1 antibody detection and effectively solving the problems such as outcome and prognosis of HCV diseases and prediction of interferon curative effect and has extremely great clinical application prospects.
Owner:山东莱博生物科技有限公司

Hepatitis C virus RNA (ribonucleic acid) extracting kit

The invention relates to a hepatitis C virus RNA extracting kit, belongs to the field of biomedical detection and relates to the nucleic acid isolation technology, particularly to the hepatitis C virus RNA extracting technology. By combining guanidine hydrochloride with a citric acid buffer system, the hepatitis C virus RNA extracting kit achieves the technical effects of effectively extracting hepatitis C virus RNA in samples in high security, high sensitivity and high stability.
Owner:SICHUAN MACCURA BIOTECH CO LTD

Antiviral Peptide Against Hepatitis C Virus

Disclosed herein is a small peptide, LaR2C, corresponding to the C terminus of RRM2 of the human La protein that binds to the IRES element of hepatitis C virus RNA and its derivatives. This invention demonstrates that human La protein interacts with the HCV IRES element both in vitro and in vivo and also shown that this interaction enhances the efficiency of viral RNA translation (Pudi et al, J of Biol Chem, 2003). La protein has three putative RNA recognition motifs (RRM1-3). It has been established that RRM2 binds with high affinity around the GCAC sequence near the initiator AUG and the binding induces a conformational change in the HCV IRES which is critical for the internal initiation of translation (Pudi et al, J of Biol Chem, 2004).
Owner:DAS SAUMITRA +2

Detection method for mutation in 93rd amino acid of hepatitis C virus NS5A protein, and detection kit for mutation in 93rd amino acid of hepatitis C virus NS54 protein

A method for detecting a mutation in an amino acid at position 93 of a hepatitis C virus NS5A protein, the method including:synthesizing cDNA using, as a template, hepatitis C virus RNA in a sample; andperforming a real-time PCR with a cycling probe method using, as a template, the cDNA;wherein a primer set used in the real-time PCR is a certain primer set; andwherein probes used in the real-time PCR include certain probes.
Owner:SAITAMA MEDICAL UNIVERSITY

Oligonucleotide primer for effective detection of hepatitis C virus (HCV) and its use

Described herein are methods and kits for the detection of hepatitis C virus RNA is biological samples obtained from human subjects. The invention includes novel amplification primers and probes useful in the amplification of DNA derived from hepatitis C virus RNA, and kits and methods which incorporate the novel primers.
Owner:ORTHO-CLINICAL DIAGNOSTICS

In vitro activity measuring method for hepatitis C virus RNA depending RNA polymerase and application thereof

The invention provides a detecting method and an application of the extraneous activity of the RNA polymerase relied on by the hepatitis C virus RNA, which relates to an secure and practical establishing method for the detection of the activity extraneous detection of the RNA polymerase relied on by the hepatitis C virus RNA, and the application relates to the genetic engineering technology and the nanometer segregation enrichment and RNA related technology field. The invention expresses the soluble NS5B protein through the utilization of the genetic engineering technology, and prepares the nanometer magnetic particle. One end of a RNA template is fixed on the nanometer magnetic particle, and added in the 96 hole reaction plate micromesh, and added with NS5B protein to compose a RNA duplication system, and a duplicated RNA double chain on the nanometer magnetic particle is mixed with marked biological elements. After the RNA is duplicated, the nanometer magnetic particle is attached at the micromesh bottom through an external magnetic field magnet, after repeatedly cleaning and attaching, the nanometer magnetic particle is combined with the marked combining factor of the horse radish peroxidase, after attaching and cleaning, the substrate constant of the horse radish peroxidase is added to develop color. New NS5B protease activity inhibitor can be screened from a traditional Chinese storeroom and a compound storeroom by the established method.
Owner:李越希

Detection method for mutation in 93rd amino acid of hepatitis c virus ns5a protein, and detection kit for mutation in 93rd amino acid of hepatitis c virus ns5a protein

A method for detecting a mutation in an amino acid at position 93 of a hepatitis C virus NS5A protein, the method including:synthesizing cDNA using, as a template, hepatitis C virus RNA in a sample; andperforming a real-time PCR with a cycling probe method using, as a template, the cDNA;wherein a primer set used in the real-time PCR is a certain primer set; andwherein probes used in the real-time PCR include certain probes.
Owner:SAITAMA MEDICAL UNIVERSITY

Truncated hepatitis C virus hcv NS3 antigen and its preparation and application

The invention discloses a truncated type hepatitis c virus HCV NS3 antigen which is a nonstructural protein amino acid sequence of the NS3 full-length gene encoded 1252th-1526aath segment in an HCV genome. The amino acid sequence is shown as SEQ ID NO.1, and a corresponding nucleotide sequence is shown as SEQ ID NO.2. A preparation method of HCV NS3 recombinant antigen protein comprises the steps that an optimized escherichia coli codon for expressing the truncated type hepatitis c virus HCV NS3 antigen are established, the nucleotide sequence of the codon is shown as SEQ ID NO.3, the gene segment of the codon undergoes double enzyme digestion and then is connected with an expression vector undergoing the enzyme digestion as well to obtain a recombinant expression plasmid, the antigen can perform expression by adopting a conventional method, and the antigen protein is obtained through purification. The antigen protein has high sensitivity and good specificity and is used for detecting an HCV antibody, and the coincidence rate of a detection result of a patient with positive hepatitis c virus RNA is as high as 97%. It is indicated that the antigen can be applied to polyclonal or monoclonal antibodies and preparation of hepatitis c virus detection kits and has good prospect of clinical application.
Owner:山东莱博生物科技有限公司

HCV RNA having novel sequence

A truncated form of hepatitis C virus gene obtained by deleting a portion of a region of a gene encoding NS2 protein from the core protein of hepatitis C virus while sustaining its translation frame. In particular, a gene as described above wherein the above-described portion of a region of the gene is located in a region encoding E1 protein and E2 protein.
Owner:ADVANCED LIFE SCI INST

Inhibitors of hepatitis c virus RNA-dependent RNA polymerase, and compositions and treatments using the same

The present invention provides compounds of formula (4), and their pharmaceutically acceptable salts and solvates, which are useful as inhibitors of the Hepatitis C virus (HCV) polymerase enzyme and are also useful for the treatment of HCV infections in HCV-infected mammals. The present invention also provides pharmaceutical compositions comprising compounds of formula (4), their pharmaceutically acceptable salts and solvates. Furthermore, the present invention provides intermediate compounds and methods useful in the preparation of compounds of formula (4).
Owner:PFIZER INC
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