The invention provides an inducing and preparation method of
rat liver S9. The inducing and preparation method comprises the following steps of (a) selecting a rat, injecting two types of inducing agents into an
abdominal cavity by different types and dosages according to the weight of the rat, and determining the optimum inducing method; (b) killing the rat, removing residual blood in the livers under the sterile condition, and fetching out the livers; (c) under the sterile condition, adding a pre-freezing storage buffer liquid according to a certain liver-
wet weight ratio, fully shearing, and equalizing pulp in an ice bath; (d) centrifuging a tissue liquid after pulp equalizing at
low speed, collecting a supernatant and a precipitate, centrifuging the supernatant liquid at high speed, and collecting the supernatant liquid, so as to obtain the liver S9 for the first time; (e) adding the pre-freezing storage buffer liquid into the collected precipitate according to a certain ratio, fully
grinding under the sterile and ice bath conditions, crushing cells by ultrasonic
waves under different conditions, centrifuging at high speed, and collecting the supernatant liquid, so as to obtain the liver S9 again; (f) detecting the contents of
protein and
enzyme in the liver S9 by a BCA (bicinchoninic acid) method and a CO (
carbon monoxide) reduction method under the different conditions. The inducing and preparation method of the
rat liver S9 has the advantages that the output of liver S9 is increased, and the content of
enzyme P450 is obviously increased.