The invention relates to an iris segmentation method using Purkinje reflection and a dynamic three-dimensional model, which comprises the following steps of: arranging a plurality of
infrared light sources around a camera, and acquiring a plurality of frames of eye images with reflection points of a patient from a
video sequence shot by the camera; calculating an eyeball center by using the position of the reflection point and establishing an initial three-dimensional eyeball model; according to changes of the positions of the inner
canthus and the outer
canthus, the three-dimensional coordinates and the posture of the eyeball center are correspondingly adjusted, and a three-dimensional eyeball model is updated; in the updated three-dimensional eyeball model, acquiring an iris
radius according to an eyeball center, and establishing a three-dimensional iris circle equation; and projecting the three-dimensional iris circle to a two-dimensional
image plane to obtain an iris
ellipse parameter, and generating an iris segmentation result. Through constructing a dynamic three-dimensional model, eyeball
model parameters including an eyeball center, an iris
radius and an iris center are detected in combination with
deep learning, a three-dimensional space
optical axis and an iris
ellipse are calculated, and iris segmentation adapted to
head posture change and eyeball movement is realized.