The invention relates to a micro-fluidic
chip for capturing single cells / single granules through high-flux
pairing and application of the micro-fluidic
chip. The micro-fluidic
chip comprises three parts, namely a capturing layer, a
control layer and a carrier from top to bottom, wherein the capturing layer consists of two runners in parallel and a connection channel; the
control layer is an isolation pump layer; a plurality of single granule / single
cell capturing units are arranged inside the runners and are used for capturing single granules / single cells; the connection channel is used for communicating two paired capturing units; isolation channels are positioned below the connection channel, are perpendicular to the connection channel and are isolated by diaphragms. The micro-fluidic chip is capable of capturing single granules / single cells in a
high flux manner, is high in capturing efficiency, capable of achieving
pairing of single granules / single granules, single cells / single cells and single granules / single cells, high in
pairing efficiency, capable of achieving parallel operation and control on paired units, such as isolation, culture,
cracking and
nucleic acid amplification of single cells, can be widely applied to the field of
single cell analysis, and is applicable to
cell samples of small numbers.