The invention discloses a tightness-adjustable automatic operation device. For the tightness-adjustable automatic operation device, linear displacement spherosyns are arranged in guide rails, readingheads are arranged in sliding sleeves, the tightness-adjustable automatic operation device further comprises at least two manipulators and a main motor, the main motor is fixedly mounted on a bottom plate of the operation device, an output shaft of the main motor is in linkage with the sliding sleeves through a reduction gearbox and a lead screw, each manipulator is composed of a pair of mechanical arms capable of rotating in an opposite way, each mechanical arm is composed of a plurality of sections of movable joints, the movable joints are rotatably connected through rotation shafts, at least two grooves are axially symmetrically formed in each rotation shaft, a guide rod is telescopically arranged in each groove, a first contrate gear is horizontally arranged at the upper end of each guide rod, a first drive mechanism is slidingly arranged at the upper end of each mechanism arm, a second contrate gear is telescopically arranged at the upper end of each first drive mechanism, and each second contrate gear is in selective linkage with the corresponding first contrate gear. With the tightness-adjustable automatic operation device, the technical problem that the mechanical arms on the existing grabbing and climbing devices are fixed in form, consequently, the application range is limited, is solved.