37 results about "Melanoma Antigens" patented technology

The present invention provides the means and methods for selecting immunogenic peptides and the immunogenic peptide compositions capable of specifically binding glycoproteins encoded by HLA alleles and inducing T cell activation in T cells restricted by the allele. The peptides are useful to elicit an immune response against a desired antigen. The immunogenic peptide compositions of the present invention comprise immunogenic peptides having an HLA binding motif, where the peptide is from a target antigen. Target antigens of the present invention include prostate specific antigen (PSA), hepatitis B core and surface antigens (HBVc, HBVs) hepatitis C antigens, Epstein-Barr virus antigens, melanoma antigens (e.g., MAGE-1), human immunodeficiency virus (HIV) antigens, human papilloma virus (HPV) antigens, Lassa virus, mycobacterium tuberculosis (MT), p53, CEA, trypanosome surface antigen (TSA) and Her2 / neu.

The metabolization of certain phenols, monophenols or benzenediols into reactive quinone compounds, in particular ortho-quinones and related reactive intermediates, which is brought about by oxidation of monophenols and benzenediols by proteins exhibiting tyrosinase activity, such as human tyrosinase and the related proteins TRP1 and TRP2. The compounds function as haptens that become covalently bound to the tyrosinase enzymes, in particular to histidine moieties, in or near the catalytic site of proteins exhibiting tyrosinase activity, such as tyrosinase, TRP1 and TRP2. An immune response is then to be mounted against these haptenized auto-antigens to treat malignancies.

The present invention provides methods and materials related to the detection of colorectal neoplasm-specific markers (e.g., markers associated with colorectal cancer, markers associated with adenoma) in or associated with a subject's stool sample. In particular, the present invention provides methods and materials for identifying mammals (e.g., humans) having a colorectal neoplasm by detecting the presence and level of indicators of colorectal neoplasia such as, for example, long DNA (e.g., quantified by Alu PCR) and the presence and level of tumor-associated gene alterations (e.g., mutations in KRAS, APC, melanoma antigen gene, p53, BRAF, BAT26, PIK3CA) or epigenetic alterations (e.g., DNA methylation) (e.g., CpG methylation) (e.g., CpG methylation in coding or regulatory regions of bmp-3, bmp-4, SFRP2, vimentin, septin9, ALX4, EYA4, TFPI2, NDRG4, FOXE1) in DNA from a stool sample obtained from the mammal.

The present invention relates to the mannose-receptor selective lysinylated cationic amphiphile and a process for preparation thereof. The compounds of the present invention can target DNA vaccines to antigen presenting cells (APCs) such as macrophages and dendritic cells (DCs), via mannose receptors expressed on the cell surface of APCs. The cationic amphiphiles disclosed herein show enhanced cellular and humoral immune response compared to their mannosyl counterparts in genetic immunization in mice. The present invention discloses that immunization with electrostatic complexes (lipoplexes) of DNA vaccines encoding melanoma antigens (gp100 and tyrosinase) and liposome of the presently described novel lysinylated cationic amphiphiles with mannose-mimicking shikimoyl head-groups provides long-lasting (100 days post melanoma tumor challenge) protective immunity in all immunized mice. Cationic amphiphiles with mannose-mimicking shikimoyl head-groups described in the present invention are likely to find future applications in the field of genetic immunization.

The invention discloses a GM-CSF (Granulocyte-Macrophage Colony-Stimulating Factor) and MART-1 (Melanoma Antigen Recognized By T-Cells 1) dual-gene co-expression recombinant vector. The GM-CSF and MART-1 dual-gene co-expression recombinant vector is characterized that a GM-CSF gene, an IRES (Internal Ribosome Entry Sequence) and an MART-1 gene are connected in sequence along the vector transcription direction, or the MART-1 gene, IRES and GM-CSF gene are connected in sequence along the vector transcription direction; a nucleotide sequence of the GM-CSF gene is as shown in SEQ ID NO:1 in a sequence table; the nucleotide sequence of the MART-1 gene is as shown in SEQ ID NO:2 in the sequence table; the nucleotide sequence of the IRES is as shown in SEQ ID NO:3 in the sequence table. According to the dual-gene co-expression recombinant vector, the IRES sequence is utilized for connecting the GM-CSF gene with the MART-1 gene, and thus human melanoma differentiation antigen and the granulocyte-macrophage colony-stimulating factor can be expressed in the same vector at the same time; the recombinant vector can be applied to immunogene therapy of melanoma, enables the immune regulation effect of cytokines to be played, and can also produce specific anti-tumor effect for malignant melanoma in a targeting way.

The invention relates to an anti-malignant melanoma vaccine composition, which is prepared by mixed culture of antigen mixture and dendritic cell culture, wherein the antigen mixture includes malignant melanoma antigen, tuberculosis antigen and / or rod-shaped body. The vaccine composition can better stimulate the proliferation of specific T cells in the body, and has a very strong specific killing function on target malignant melanoma cells. The vaccine composition is easy to use, has a good therapeutic effect, and has a good application prospect.

The invention provides an isolated or purified T cell receptor (TCR) having antigenic specificity for a) melanoma antigen family A (MAGE A)-3 in the context of HLA-A1 or b) MAGE-A12 in the context of HLA-Cw7. The invention further provides related polypeptides and proteins, as well as related nucleic acids, recombinant expression vectors, host cells, and populations of cells. Further provided by the invention are antibodies, or an antigen binding portion thereof, and pharmaceutical compositions relating to the TCRs of the invention. Methods of detecting the presence of cancer in a host and methods of treating or preventing cancer in a host are further provided by the invention.