The invention relates to a multistage fracturing horizontal well inter-crack interval CO2 flooding oil extraction method. The method includes following steps: performing sectional fracturing on a horizontal well shaft to form multiple fracturing cracks perpendicular to the horizontal well shaft; mounting an oil pipe I and an oil pipe II in a casing in the horizontal well shaft, mounting flow controllers I at positions, corresponding to even-level cracks, of the oil pipe I, mounting flow controllers II at positions, corresponding to odd-level cracks, of the oil pipe II, and arranging a packer at a position, between the odd-level cracks and the even-level cracks, in an annular space of an oil jacket; at an initial production stage, utilizing natural energy for extraction until bottom hole pressure is lowered to bubble point pressure, and utilizing crack intervals for extraction, injecting CO2 into the oil pipe I, wherein CO2 enters the even-level cracks, and crude oil is output from the odd-level cracks and enters the oil pipe II at the same time; when oil yield is lower than economic limit yield, stopping injecting CO2. By the method, crude oil recovery rate can be increased substantially, and gas channeling can be inhibited effectively.