The invention discloses an optical interleaver with adjustable high speed isolation and centre wavelength. A single optical fiber collimator, a first polarization displacement crystal, a first half wave plate group, a first electro light delay plate, a quarter-wave plate, a double refraction crystal, a second half wave plate group, a second electro light delay plate, a third half wave plate group, a second polarization displacement crystal, a fourth half wave plate group, a ridge prism, a third polarization displacement crystal and a double optical fiber collimator are sequentially put in the direction of the same horizontal optical path; parallel beams separated by the second polarization displacement crystal are collimated by the fourth half wave plate group, the ridge prism, the third polarization displacement crystal and coupled to the double optical fiber collimator; the high speed adjustment of the channel separation of the optical interleaver is realized by controlling the first electro light delay plate; the high speed adjustment of the centre wavelength of the optical interleaver is realized by controlling the second electro light delay plate. The invention realizes the high speed adjustment of the isolation and the centre wavelength of the optical interleaver, and is characterized by the compact structure, the low cost, the stable performance, etc.