The invention provides nauclea officinalis pierre ex pitard total alkaloid extract, which comprises 3-R-3,4-dihydroangustoline, naucleofficine D, 1,2,3,4-tetrahydro-beta-carboline, 3-S-3,4-dihydroangustoline, latifoliamide D, latifoliamide B, angustoline, 3,14-dihydroangustine, 3,14,18,19-tetrahydroangustine, 6'-acetyl strictosamide, vincoside lactam and strictosamide. The content of total alkaloids in the nauclea officinalis pierre ex pitard total alkaloid extract obtained by such a preparation method is more than 50 weight percent. The nauclea officinalis pierre ex pitard total alkaloid extract obtained by the preparation method and a medicinal composition prepared from the nauclea officinalis pierre ex pitard total alkaloid extract have better anti-inflammatory effects.