The invention provides screen frame positioning equipment and a screen frame positioning method. The screen frame positioning equipment comprises a base, a worktable, a conveying device, a driving device, guide rails, positioning pillars, a dual-rod left-and-right positioning cylinder, a positioning push block, an artificial screen frame positioning device, a cross beam, a cross beam cylinder and a scraper assembly. The screen frame positioning equipment and the screen frame positioning method have the benefits that the mounting and dismounting are convenient, and a screen frame does not need to be changed; the safety is achieved, the manual positioning can be simulated, and the positioning is accurate; after power failure, the manual operation can be automatically converted; panels are called out, the overprinting is accurate, and the efficiency is high.