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System and method for integrating on-line user ratings of businesses with search engines

A computer program product is provided as a business rating system to rank business that are relevant to a given Internet search topic. Business ratings are stored in a ranking repository that can be optionally searched by the user along with a user-defined search engine query. The business ratings are compiled from on-line questionnaires attached to the search engine results and/or on-line surveys obtained through other web based rating services. The business ratings assess the quality of the businesses in terms of “interactive” criteria such as customer satisfaction, professionalism, and cost and ease of use of the businesses' products or services. The business rating system is comprised of an on-line indexing engine, a query transformer, a search results transformer, a ranking based result sorter, an on-line ranking system, a metadata repository, and an on-line ranking repository. The business rating system integrates the ratings with the search results, and ranks the search results based on such business ratings. In this manner, the user of a search engine receives feedback from other users about businesses of interest. Eventually, businesses with higher ratings will be ranked at the top of the search list, while business with lower ratings will be ranked lower.
Owner:IBM CORP

Method of detection of DNA end(s) and its use

The invention concerns the method of detection of DNA end(s) in a biological material, comprising the following steps I III and at least one of sub-steps a-h of each of steps I-III: I. PREPARATION OF THE MATERIAL comprising a fixation and/or permeabilization and/or lysis and/or isolation and/or fractionation and/or immobilization of the biological material, b. increasing accessibility of DNA end(s), c. blocking nonspecific binding site(s) for molecules type 2-6, in the biological material; H. PROCESSING OF DNA END(S) comprising d.): modification of DNA end(s) by chemical or physical processing followed by binding molecules type 1 to the DNA end(s) by catalytic or noncatalytic means; blocking nonspecific binding site(s) for molecules type 2-6 in the biological material; III RECOGNITION AND DETECTION OF THE MODIFIED DNA END(S): incubation of the biological material from step II with at least two molecules type 2 and 3 which bind to the molecules type 1 in a manner that allows steps leading to rolling circle amplification (RCA) reactions, g. detection of DNA end(s) by: i. optionally contacting suitable molecules type 4 and/or 5 with molecules type 2 and 3, wherein the molecules type 4 and/or 5 are conjugated with the oligonucleotides type 1, ii. adding oligonucleotides type 2 and enzyme ligase to allow hybridization of said added oligonucleotides type 2 to the oligonucleotides type 1 already linked to molecules type 4 and/or 5, or to molecules type 2 and 3 if they are linked to oligonucleotides type 1, and subsequently performing DNA ligation of oligonucleotides type 2, iii. performing amplification by adding enzyme polymerase and a solution of nucleotides to allow rolling circle amplification (RCA) reactions, and molecules type 6 to allow subsequent hybridization of molecules type 6 to thus obtained product of RCA reactions, h. detection of molecules type 6; wherein when more than one sub-step a-c of step I is performed then they may occur in any order. The invention concerns also use of rolling circle replication for marking the presence and position of single DNA end(s) and use above-mentioned method for detection of DNA end(s) in a biological material.
Owner:INTODNA SPOLKA AKCYJNA
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