The invention provides a gear shifting force detection positioning and clamping device for a multi-type shifter assembly. A positioning assembly is based on the positioning principle of being provided with two pins on each face, and a coarse positioning mode and a fine positioning mode are combined for positioning. The positioning assembly comprises two positioning plates, two positioning blocks, two type distinguishing detection bars and two positioning in-place detection probes, wherein the two positioning plates and the two positioning blocks are used for conducting coarse positioning. When a shifter is fixed on the positioning assembly, the approximate position of the shifter is fixed preliminarily through the positioning plates and the positioning blocks first, then the two detection bars and sensors fixed below the detection bars can act to judge the type of the shifter, meanwhile, the two positioning in-place detection probes with springs are pressed downwards by the shifter, distance change happens between the positioning in-place detection probes and sensors fixed below the positioning in-place detection probes, and in this way, whether the shifter is fixed in place or not can be judged. The positioning assembly is a detachable module type positioning assembly, and different positioning assemblies can be correspondingly used according to the requirements for different types of shifters.