The invention discloses an efficient culture medium for pleurotus eryngii and a preparation method of the culture medium, and relates to the technical field of pleurotus eryngii culture media. The culture medium is prepared from 45-55 parts of tea dregs, 35-45 parts of cassava residues, 30-35 parts of sustained release granules, 20-30 parts of distillers' grains, 15-20 parts of oyster shells, 15-20 parts of amylase, 15-20 parts of protease, 10-15 parts of expanded vermiculite, 10-15 parts of soybean cakes, 5-10 parts of rice hull powder, 5-10 parts of bran and an appropriate amount of water. Accordingly, the nutritional ingredient content is high, the produced pleurotus eryngii is high in weight, rapid in growth, high in disease resistance, soft in pulp quality and good in taste.