The invention discloses an optimization method based on dynamic characteristic I-shaped beam section design. The method comprises the steps that deformation potential energy and kinetic energy of an I-shaped beam bridge are obtained at first, then, an energy variation method is utilized for obtaining a governing differential equation and natural boundary conditions of I-shaped beam vibration, a relevant application program is compiled based on this, and fine analysis is carried out on natural vibration characteristics of the structure through Matlab software; finally, the inherent frequency value of the I-shaped beam bridge is used as the criterion, in other words, the inherent frequency value is large, structure deformation potential energy is small, and the I-shaped beam section size is optimal, and the effectiveness of the method is further proved through the structure statics analysis. Due to the fact that mechanical analysis is accurate, the I-shaped beam bridge with the optimized section size is in a good mechanical state, and defects such as beam cracking, stiffness degradation and midspan excessive downwarping can be avoided. The optimization method is clear in mechanical conception and simple in calculation, has a good application value, and is the beneficial supplement for an existing I-shaped beam bridge design theory.