The invention relates to the technical field of air purification, and particularly discloses a preparation method of a negative oxygen ion release liquid, which comprises: 1) processing negative oxygen ion powder to achieve a particle size of 300 nm, mixing into water under a stirring condition with a weight ratio of the negative oxygen ion powder to the water of 1: 20, carrying out standing precipitation, filtering, and removing large-particle solid particles; and 2) adding a defoaming agent, a thickening agent, a preservative, an anti-freezing agent and a silver ion antibacterial agent, fully stirring for 10 hours, then filtering, and removing large solid particles to obtain a finished product. Negative oxygen ions generated by the prepared negative oxygen ion release liquid can reach 5000-10000 ions/cm < 3 >, the negative oxygen ion release amount is high, air is effectively purified and refreshed, pm2.5, pm10 and harmful particles floating in the air are settled, meanwhile, pollutants such as formaldehyde, ammonia, benzene and Tvoc and peculiar smells such as acid odor and smoke odor in daily life can be efficiently removed. The removal rate of formaldehyde and ammonia reaches90% or above, the radiation of the product is far lower than the national standard, and the product is environment-friendly and safe.