The invention provides a non-contact EMAT (Electromagnetic Acoustic Transducer) detection method and system for a micro-mass sensor. According to the invention, an electrostatic magnetic field is generated by magnets, sinusoidal alternating current is introduced into an excitation coil, a resonance piece is placed above the excitation coil, and resonance is generated when the frequency of the excitation coil is the same as the intrinsic frequency of the resonance piece, so that a secondary alternating magnetic field is induced, and in induction current is generated in a differential detectioncoil; and when a minute amount of substance to be measured is adsorbed to a sensitive thin layer covered on the upper surface of the resonance piece, the resonant frequency of the resonance piece drifts, and the substance to be measured is quantitatively measured through calculating the drifting amount of the resonant frequency. The EMAT is introduced, the acoustic vibration is excited and detected by using electromagnetic induction so as to realize a non-contact scheme, complex structures such as a micro-cantilever beam structure are replaced by the simple structure, and the absolute frequency shift is replaced by the relative frequency difference between different modes, thereby reducing disturbance caused by the external environment, and realizing high-precision measurement for the micro-mass substance.