A
single cell sequencing data interpolation method based on a
biclustering and graph
convolutional neural network comprises the following steps: 1) respectively downloading matrix-form
gene expression data of human and mouse from a
gene database, and screening out cells of which the total number of
gene expressions is less than 3 and genes of which the total number of
cell expressions is less than 200; 2) performing
feature selection on the
gene expression matrix, finding out cells with consistent growth trend, forming a new two-dimensional matrix, and constructing a
cell connection diagram; 3) constructing a BiGCN single-
cell interpolation model by using a graph
convolutional neural network, learning a nonlinear relationship between cells, dividing a
data set into a
training set and a
test set, training the model by using the
training set, and reserving the trained BiGCN single-cell interpolation model; 4) inputting the
test set into the trained BiGCN single cell interpolation model to obtain an interpolation result, and evaluating the interpolation result by using an evaluation index; the method has the characteristics that the
feature extraction capability is improved, interference of redundant zero expression is avoided, data sparsity is effectively relieved, and the interpolation accuracy is improved.