The invention provides a
system and a method for
differential phase contrast
microscopy. The
system comprises a light strength modulating module, a light focusing lens, an objective lens and an imageacquisition module. The light strength modulating module is used for modulating an incident
light field to a detecting
optical field with
gradient strength distribution according to a
control signal.The focusing lens is used for receiving the detecting
optical field and generates an off-axis
optical field for projecting to a to-be-measured object, and furthermore generates the
light field of theto-be-measured object for receiving by the objective lens. The
image acquisition module is used for receiving the optical field of the to-be-measured object, thereby generating an
optical image with corresponding strength gradient distribution. The method further comprises the steps of controlling the light strength modulating module for generating a first gradient shield with gradually reducing light strength in a first axial direction, a second gradient shield with gradually increasing light strength in the first axial direction, a third gradient shield with gradually reducing light strengthin a second axial direction, and a fourth gradient shield with gradually increasing light strength in the second axial direction, and making the
image acquisition module to acquire a first
optical image, a second
optical image, a third optical image and a fourth optical image which respectively correspond with the first gradient shield, the second gradient shield, the third gradient shield and the fourth gradient shield.