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35 results about "Polymyxin B Sulfate" patented technology
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The sulfate salt form of polymyxin B, a mixture of the polypeptides polymyxins B1 and B2, both obtained from Bacillus polymyxa strains, with antimicrobial activity. Polymyxin B exerts its antimicrobial effect through its cationic detergent action on cell membranes. Specifically, this antibiotic binds to the negatively charged site in the lipopolysaccharide layer of the bacterial cell membrane via electrostatic affinity with the positively charged amino groups in the cyclic peptide portion. Subsequently, the fatty acid portion of polymyxin B dissolves in the hydrophobic region of the bacterial cell membrane. This results in an alteration in cell membrane structure, disruption of cell wall integrity and an increase in permeability for water and molecules. This will eventually lead to bacterial cell death.
Method of treatment of otitis externa
This invention relates to a method of treating otitis externa using a topical combination medication, including one or more antifungal agent such as, for example fluconazole, voriconazole, itraconazole, clotrimazole, amphotericin B, caspofungin, micafungin, terbinafine, naftifine, butenafine, amorolfine, ravuconazole, posaconazole, flucytosine, econazole, enilaconazole, miconazole, oxiconazole, saperconazole, sulconazole, terconazole, tioconazole, nikkomycin Z, anidulafungin (LY303366), nystatin, pimaricin, griseofulvin, ciclopirox, haloprogin, tolnaftate, and undecylenate and one or more antibacterial agent such as neomycin sulfate, polymyxin B sulfate, colistin sulfate, gentamycin, tobramycin, chloramphenicol, Ciprofloxacin, Ofloxacin, a penicillin compound, a cephalosporin compound, a macrolide compound, a fluoroquinolone compound, streptomycin, or kanamycin.
Owner:FAIRFIELD CLINICAL TRIALS
Photodynamic therapy utilizing multiple duty cycle light modulation
InactiveUS20060234959A1Improve efficiencyGood curative effectBiocideCavity massageCancer cellLight modulation
A method of photodynamic disruption of target cells within a target cell site wherein the target cells are associated with one or more of a sterilization procedure, a biofilm eradication procedure, a sterilization procedure of a medical prosthesis, a treatment of an infection at a tissue site, eradication of cancer cells and a fluid or food decontamination process. Surface acting agents such as benzalkonium chloride or polymyxin B sulfate or both may be utilized. A controllable light source capable of delivering light at a plurality of duty cycles is utilized to cause photoactivation of the photosensitive material resulting in degradation of target cells within the site.
Owner:ADVANCED PHOTODYNAMIC TECH
Method for extracting polymyxin B from fermentation broth
InactiveCN103130875AAvoid infectionLower resistanceAntibacterial agentsPolymyxinsTime-ConsumingChemistry
The invention discloses a method for extracting polymyxin B from a fermentation broth of the polymyxin B. The method for extracting the polymyxin B from the fermentation broth of the polymyxin B comprises the following steps: a, conducting solid-liquid separation on the fermentation broth of the polymyxin B and obtaining a filtrate; b, extracting the filtrate with a polar organic solvent, and extracting an extract liquor reversely with water when the extract liquor is at the potential hydrogen (pH) value of 1.0-2.0; c, adding the reversal extract liquor which is obtained from the step b into inorganic salt, wherein the volume of the reversed extract liquor of the inorganic salt is 3-8% (w / v), and adjusting the pH value to be 9.0-10.0 so that the polymyxin B is obtained after precipitation and sediment. Preferably, the method for extracting the polymyxin B from the fermentation broth further comprises a step of d: reacting the polymyxin B and sulfuric acid to form salt, and dissolving and precipitating, thereby obtaining the polymyxin B sulfate. The polymyxin B or the sulfate prepared by means of the method is higher in yield and purity, the purity of a product is above 85%, the tilter is above 8100IU / g, and the yield is above 60%. The method for extracting polymyxin B from the fermentation broth has the advantages of being easy and convenient to operate, short in time consuming, low in requirement of equipment, and capable of recycling the organic solvent and being used for large-scale industrial production.
Owner:SHANGHAI INST OF PHARMA IND +1
Ointment composition for treating decubitus ulcers and methods for its making and its use
ActiveUS7655717B2Simple and inexpensive to manufactureEasy to useBiocideInorganic boron active ingredientsAdditive ingredientDimethyl siloxane
An ointment composition for treating decubitus ulcers and methods for its making and its use. The composition includes a skin protestant ointment, a rash cream, an antibiotic ointment, virgin olive oil, and boric acid powder. The skin protestant ointment includes active ingredients petroleum 53.4%, lanolin 15.5%, and inactive ingredients cod liver oil containing vitamin A & vitamin D, a fragrance, light mineral oil, microcrystalline wax, and paraffin. The rash cream includes active ingredients dimethicone 1% and zinc oxide 10%, and inactive ingredients aloe barbadensis extract, benzyl alcohol, coconut oil, cod liver oil containing vitamin A & vitamin D, a fragrance, glycerol oleate, light mineral oil, ozokerite, paraffin, propylene glycol, sorbitol, synthetic beeswax, and water. The antibiotic ointment includes active ingredients polymyxin B sulfate 5,000 units, bacitracin zinc 400 units, and neomycin base (as sulfate) 3.5 mg., and an inactive ingredient white petroleum.
Owner:GOULBOURNE MARY J
Composite hydrogel wound dressing and preparation method thereof
The invention provides a composite hydrogel wound dressing and a preparation method thereof. The aminated collagen and oxidized sodium alginate are used as base materials; two polypeptide antibiotics,namely polymyxin B sulfate and bacitracin are loaded; and the AC / OSA-PB composite hydrogel dressing is prepared through a Schiff base reaction. The preparation method is simple to operate; the requirements on reaction conditions are low; gel can be formed in a short time, large-scale production is facilitated, and the prepared wound dressing not only has a good bactericidal effect, but also can improve the HSF cell mobility and the HSF cell proliferation rate to the maximum extent, has excellent wound healing promoting capacity, and also has mechanical properties and mechanical properties suitable for skin wounds.
Owner:FUZHOU UNIV
Culture medium for fermenting polymyxin B sulfate and method for fermenting and producing polymyxin B sulfate
ActiveCN106868079ABiological metabolic pathways are smoothGeneration of effective regulationBacteriaMicroorganism based processesAmylaseDipotassium phosphate
The invention provides a culture medium for fermenting polymyxin B sulfate. Each 100ml of the culture medium is prepared from the following components: 6g to 12g of flour, 0.03g to 0.06g of high-temperature amylase, 0.4g to 2g of cottonseed meal, 0.3g to 1g of corn pulp, 0.01g to 0.05g of dipotassium phosphate, 0.2g to 0.6g of ammonium sulfate, 0.2g to 0.6g of calcium carbonate, 0.02ml to 0.08ml of a de-foaming agent and the balance of water. A method for fermenting and producing the polymyxin B sulfate comprises the following the steps: inoculating the culture medium with strain seed liquid for producing the polymyxin B sulfate; fermenting and culturing fermentation liquid at 26 DEG C to 32 DEG C, wherein the pH (Potential of Hydrogen) value is 6.7 to 7.4 at the beginning of fermenting; controlling the pH value of the fermentation liquid to be natural during a fermentation process, wherein the dissolved oxygen volume concentration of the fermentation liquid is 10 percent to 30 percent. All the components of the culture medium are cooperatively matched and the content of impurities B2P is controlled to be lower than 1.5 percent.
Owner:SHANDONG LUKANG PHARMA
Polymyxin sulfate B monoclonal antibody hybridoma cell strain and application thereof
InactiveCN110923208AImprove featuresHigh detection sensitivityBiological material analysisImmunoglobulins against bacteriaImmuno detectionMicrobiological culture
The invention discloses a polymyxin sulfate B monoclonal antibody hybridoma cell strain and application thereof, and belongs to the field of food safety immunodetection. The polymyxin sulfate B monoclonal antibody hybridoma cell strain OVA17 is obtained through screening and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.18516. The monoclonal antibody secreted by the cell strain has good specificity and detection sensitivity (IC50 value is 13.13 ng / mL) for polymyxin B sulfate, can realize detection of polymyxin B sulfate residue in feed and livestock and poultry food, provides a raw material for immunodetection of polymyxin B sulfate residue in food. Practical application value is achieved.
Owner:JIANGNAN UNIV
Moisturizing skin ointment - composition consisting of polymyxin B Sulfate, bacitracin zinc, neomycin (the combination of which totals 1 ounce), hydrocortisone acetate (1 ounce) and white petrolatum (13 ounces)
This invention relates to a moisturizing skin ointment composition consisting of polymyxin B Sulfate, bacitracin zinc, neomycin (the combination of which totals 1 ounce), hydrocortisone acetate (1 ounce) and white petrolatum (13 ounces). When combined and water evaporated, an external ointment that penetrates the skin and alleviates dry skin conditions, itching and minor scrapes and scratches is created. The properties of the antibiotic products help to promote healing of minor scrapes and scratches as well as preventing the spread of dry skin conditions. The properties of the hydrocortisone aids in the alleviation of itching associated with dry skin. Because this ointment penetrates the dermis almost immediately, the moisturizing properties of petrolatum allows the full benefit of the antibiotic products and hydrocortison to remain on / in the skin through several washings thereby alleviating the need to reapply several times a day.
Owner:MEEKS JOYCE ANN
Antimicrobial hydrogel wound dressing
The antimicrobial hydrogel wound dressing is a swellable polymer gel made from about 7-9% (wt / vol) polyvinyl alcohol (PVA), preferably 8.9%, about 0.1% (wt / vol) polyvinyl pyrrolidone (PVP), and about 1-2% (wt / vol) agar, preferably 1%, the balance (about 90%) being distilled water, the foregoing contents being crosslinked by gamma radiation at a dose of about 30 kGy. Prior to crosslinking by gamma radiation, an effective amount of a pair of antibiotics is added to the gel at room temperature. The antibiotics include about 10,000 IU of polymyxin B sulfate, and about 5 mg neomycin per gram of gel. The polymyxin provides effective protection against various forms of gram negative microorganisms, and the neomycin is a broad spectrum antibiotic that provides protection against various forms of gram positive microorganisms. The hydrogel has sufficient mechanical strength for use as a wound dressing, and is capable of absorbing water up to 900% of its volume.
Owner:KING SAUD UNIVERSITY
Preserving fluid suitable for preserving virus samples at normal temperature and preparation method thereof
PendingCN112314593AConducive to preservationActiveDead animal preservationDipotassium hydrogen phosphateSodium bicarbonate
The invention discloses a preserving fluid suitable for preserving virus samples at normal temperature and a preparation method thereof. The preserving fluid comprises 150-250 g of sodium chloride, 8-12 g of potassium chloride, 20-30 g of D-glucose, 3-5 g of sodium hydroxide, 0.4-0.6 g of phenol red, 2-4 g of dipotassium hydrogen phosphate, 1-1.5 g of disodium hydrogen phosphate, 8-9 g of sodium bicarbonate, 4-5 g of bovine serum albumin, 0.5-0.9 g of 2-methyl-4-isothiazoline-3-ketone hydrochloride and 0.1-0.5 g of polymyxin B sulfate. The invention relates to the technical field of clinical examination. According to the preserving fluid suitable for preserving virus samples at normal temperature and the preparation method thereof, the prepared preserving fluid has good preserving capability, can ensure that a sample virus still has activity after preservation for more than one week at normal temperature and can be preserved at 2-8 DEG C for a longer time, does not interfere a PCR detection result, has good antibacterial capability, has low requirements on production and use environments, does not need irradiation sterilization, is low in cost and easy to mass-produce, guarantees no decomposition of nutrients, and guarantees the stability of the pH value in cooperation with an HEPES buffer solution or a phosphate buffer solution.
Owner:武汉尚飞生物科技有限公司
Impurity of polymyxin B sulfate and preparation method thereof
ActiveCN109206486AQuality improvementEnsure safety and effectivenessPolymyxinsPeptide preparation methodsImpurityPolymyxin B Sulfate
The invention discloses an impurity D17 of polymyxin B sulfate and a separation and preparation method thereof. The growth of the impurity under different conditions of temperature and pH is investigated. The preparation method provides a guarantee to control the content of the impurity, optimize the industrial production of the polymyxin B sulfate and improve the quality of a medicine.
Owner:HANGZHOU ZHONGMEI HUADONG PHARMA
Ointment preparation as well as preparation method and application thereof
InactiveCN106075394ARelieve painImprove complianceAntibacterial agentsOrganic active ingredientsTreatment effectAntibiotic Y
The invention provides an ointment preparation as well as a preparation method and application thereof, belongs to the technical field of medicine preparations, and is used for solving the problem that a conventional skin injury medicine cannot have take effects of bleeding stopping, pain relieving, infection prevention and the like into account all generally. The ointment preparation comprises a matrix, polymyxin B sulfate, neomycin sulfate, bacitracin and hemostatic, wherein the polymyxin B sulfate, the neomycin sulfate, the bacitracin and the hemostatic are uniformly dispersed in the matrix. Due to synergism of multiple antibiotics in the ointment preparation, not only is a complementary antibacterial spectrum provided, but also the antibacterial range is widened, most skin bacterial infection pathogenic bacteria are covered, and moreover, due to the synergistic and overlapping effect of the antibiotics, the antibacterial function can be enhanced, and induced drug resistance can be avoided. In addition, the ointment preparation comprises local anesthetic and hemostatic, so that a prevention and treatment effect on partial skin bacterial infection can be achieved, functions of pain relieving and bleeding stopping of partial skin wound can be achieved, and patients can be greatly relieved from pain.
Owner:ZHEJIANG REACHALL PHARMA
Method for preparing polymyxin B sulfate for injection
ActiveCN108309945AReduce the use of security risksImprove stabilityAntibacterial agentsPowder deliveryMillion UnitsInjections water
The invention relates to a method for preparing polymyxin B sulfate for injection. The auxiliary material of the prepared polymyxin B sulfate for injection is injection water only, and the specification of the polymyxin B sulfate for injection is 0.5 million units. The polymyxin B sulfate for injection, prepared through the method, has significantly improved quality and stability, and has significantly reduced insoluble particle quantity.
Owner:湖北美林药业有限公司
A kind of polymyxin b sulfate production strain, preparation method and application of polymyxin b sulfate
ActiveCN111100823BStrong ability to produce polymyxin B sulfateImprove abilitiesBacteriaMutant preparationBiotechnologyMetabolite
The invention belongs to the technical field of biological fermentation, and in particular relates to a polymyxin B sulfate production strain, a preparation method and application of polymyxin B sulfate. The present invention obtains a polymyxin B sulfate production strain through mutagenesis breeding, and optimizes the carbon source and nitrogen source of the fermentation medium, precisely regulates the fermentation conditions such as the inoculum amount, fermentation temperature, fermentation time, and tank pressure, and controls the The metabolite components finally utilize the bacterial strain obtained by mutagenesis of the present invention to produce polymyxin B sulfate in a tank unit of 2.0 g / L, exceeding the industry average level by 20%. The proportions of several impurity peaks specified by the USP / EP Pharmacopoeia in the fermented liquid in tanks are far lower than the levels recorded in domestic literature. Separation and purification of the fermentation broth to obtain high-purity and high-content polymyxin B sulfate, the biometric titer of the finished product is greater than 8100u / mg, the purity and content are both greater than 90%, the single impurities and components are all qualified, and the total impurities are less than 8 %, product quality is much higher than USP / EP standard.
Owner:黄石曼菲特生物科技有限公司
Compound ointment preparation and preparation method thereof
InactiveCN108434437AAntibacterial agentsOrganic active ingredientsButylated hydroxytolueneMillion Units
The invention provides a compound ointment preparation. According to the compound ointment preparation, per kilogram of the compound ointment preparation is prepared from the following components in parts by weight: 250-1000 million units of polymyxin B sulfate, 300-400 million units of neomycin sulfate, 25-75 million units of bacitracin, 30-50 g of lidocaine hydrochloride and a matrix added to the prescription dosage, wherein the matrix is prepared from one or more of white vaseline, yellow vaseline, ozocerite, liquid paraffin, alpha-cyclodextrin, modified chitosan, propylene glycol, methyl parahydroxybenzoats and butylated hydroxytoluene.
Owner:ZHEJIANG REACHALL PHARMA
Methods and Compositions For Topical Wound Treatment
InactiveUS20070110797A1Convenient treatmentSafe and effective amountTetracycline active ingredientsInorganic non-active ingredientsWound dressingGram
Wound Dressings for improved treatment of wounds on a patient. A wound dressing having a bandage and a healing composition. The healing composition includes from about 5 mg to about 40 mg lidocaine hydrochloride per gram of the healing composition, from about 250 units to about 2000 units polymyxin B sulfate per gram of the healing composition, and from about 10 units to about 100 units bacitracin zinc per gram of the healing composition. Article of manufacture having a packaging material and the healing composition. The packaging material includes a label indicating composition is useful for treating a patient having a skin wound and that local administration of the composition enhances healing of a skin wound.
Owner:TIPPETT ALETHA
Method for improving fermentation level of polymyxin B sulfate
ActiveCN112553277AAvoid destructionQuality improvementBacteriaMicroorganism based processesBiotechnologyInorganic salts
The invention discloses a method for improving the fermentation level of polymyxin B sulfate. The method comprises a fermentation formula and a fed-batch fermentation process, wherein the fermentationformula is divided into a basic formula consisting of a fermentation carbon source and inorganic salt and a fed-batch formula consisting of a fermentation nitrogen source; and the fed-batch fermentation process comprises the following steps: a) performing individual disinfection and sterilization on a fermentation tank; b) performing individual disinfection and sterilization on a fed-batch bottle; and c) supplementing fed-batch liquid consisting of the fermentation nitrogen source at a constant speed in the first and middle stages of fermentation. The fermentation titer of the polymyxin B sulfate obtained by fermentation culture by the method can reach 11000u / mL or above on average, the fermentation titer is improved by 30% or above compared with the original fermentation level, the component proportion of the fermentation liquor is close to the liquid phase chromatogram of a standard substance, and industrial production is easy.
Owner:NCPC NEW DRUG RES & DEV
External gel for skin wounds, and preparation method of external gel
ActiveCN112121146APromote formationHas a preventive effectAntibacterial agentsHydroxy compound active ingredientsCentella asiatica extractCollagen fibres
The invention discloses an external gel for skin wounds. The external gel is prepared from the following raw materials in parts by weight: 1-3 parts of an active component A, 4-6 parts of an active component B, 10-14 parts of a Calendula arvensis extract, 16-20 parts of a Centella asiatica extract, and 60-80 parts of an auxiliary material, wherein the active component A is at least two of polymyxin B sulfate, neomycin sulfate and bacitracin, the active component B is at least one of lidocaine hydrochloride, pramoxine hydrochloride, procaine hydrochloride and menthol, and the auxiliary materialcomprises the following raw materials in parts by weight: 0.5-3 parts of carbomer, 40-75 parts of an organic solvent, and 20-50 parts of purified water. The external gel provided by the invention hasprevention and treatment effects on the bacterial infection of skin wound parts, has analgesic and hemostatic effects on skin wounds, can promote the formation of capillaries and collagenous fibers of wound surfaces, reduces the healing days of wound surfaces, and can be used for the healing of skin wounds such as burn wounds, incised wounds and scald wounds, the healed skin is smooth and free ofscars, and thereby the healing effect is good.
Owner:SHANDONG RUIAN PHARMA CO LTD
Method for extracting polymyxin B from fermentation broth
InactiveCN103130875BHigh yieldYield preparations are improved with high purityAntibacterial agentsPolymyxinsBiotechnologyInorganic salts
Owner:SHANGHAI INST OF PHARMA IND CO LTD +1
Culture medium for fermenting polymyxin b sulfate and method for producing polymyxin b sulfate by fermentation
ActiveCN106868079BBiological metabolic pathways are smoothGeneration of effective regulationBacteriaMicroorganism based processesBiotechnologyAmylase
The invention provides a culture medium for fermenting polymyxin B sulfate. Each 100ml of the culture medium is prepared from the following components: 6g to 12g of flour, 0.03g to 0.06g of high-temperature amylase, 0.4g to 2g of cottonseed meal, 0.3g to 1g of corn pulp, 0.01g to 0.05g of dipotassium phosphate, 0.2g to 0.6g of ammonium sulfate, 0.2g to 0.6g of calcium carbonate, 0.02ml to 0.08ml of a de-foaming agent and the balance of water. A method for fermenting and producing the polymyxin B sulfate comprises the following the steps: inoculating the culture medium with strain seed liquid for producing the polymyxin B sulfate; fermenting and culturing fermentation liquid at 26 DEG C to 32 DEG C, wherein the pH (Potential of Hydrogen) value is 6.7 to 7.4 at the beginning of fermenting; controlling the pH value of the fermentation liquid to be natural during a fermentation process, wherein the dissolved oxygen volume concentration of the fermentation liquid is 10 percent to 30 percent. All the components of the culture medium are cooperatively matched and the content of impurities B2P is controlled to be lower than 1.5 percent.
Owner:SHANDONG LUKANG PHARMA
Methods and Compositions For Topical Wound Treatment
InactiveUS20070104770A1Convenient treatmentSafe and effective amountBiocideTetracycline active ingredientsMedicineGram
Methods for improved treatment of wounds on a patient. Method includes locally administering a healing composition to the skin wound such that the healing of the skin wound is enhanced. The healing composition includes from about 5 mg to about 40 mg lidocaine hydrochloride per gram of the healing composition, from about 250 units to about 2000 units polymyxin B sulfate per gram of the healing composition, and from about 10 units to about 100 units bacitracin zinc per gram of the healing composition.
Owner:TIPPETT ALETHA
Drug for treating fungal infection, and preparation method thereof
ActiveCN103933545BImprove antibacterial propertiesGood curative effectAntibacterial agentsSenses disorderBULK ACTIVE INGREDIENTCompanion animal
The invention discloses a drug for treating fungal infection of a pet, and a preparation method thereof. The drug comprises the following components in percentage by mass: 5.86-13.4% of active ingredients, 10-24% of mineral oil, 12-30% of propylene glycol, 10-24% of glycerinum, 3-12% of DC-345, 0.5-2% of sodium chloride, 0.5-6% of EM-90, 2-10% of R9331, and the balance of water, wherein the active ingredients comprise 1.2-2.0% of nystatin, 0.4-0.8% of neomycin sulfate, 0.2-0.5% of polymyxin B sulfate, 0.06-0.1% of triamcinolone acetonide, and 4%-10% of lidocaine sulfate. The invention further provides the preparation method of the preparation. The drug is prepared by matching the components through a specific proportion and mainly aims at common skin diseases caused by various fungal infections of pet dogs and pet cats and symptoms of wound infection fester or long-time non-cicatrization caused by trauma.
Owner:SHENZHEN REDRAY BIOTECHNOLOGY CORP LTD
Compound oxytetracycline oculentum for pets and preparation method thereof
PendingCN113786475AClear ingredientsEasy to useSenses disorderTetracycline active ingredientsOfficinalOxytetracycline Hydrochloride
The invention provides a compound oxytetracycline oculentum for pets, the compound oxytetracycline oculentum comprises, by mass, 0.5-5% of an active component and 95-99.5% of an oculentum matrix, the active component comprises oxytetracycline hydrochloride and polymyxin B sulfate, and the oculentum matrix comprises albolene and liquid paraffin. The raw materials and the auxiliary materials are medicinal, the quality is easy to control, and the preparation is convenient; the active ingredients are anti-infection western medicine ingredients which are clear and are safer than some complex traditional Chinese medicine ingredients; and the compound oxytetracycline oculentum for pets has an obvious effect on treating eye infection of pets.
Owner:南京联智医药科技有限公司
Kit for separating Escherichia coli in urine and preparation method thereof
PendingCN114814212AIncrease concentrationHigh sensitivityBiological material analysisEscherichia coliMagnetic bead
The invention discloses a kit for separating Escherichia coli in urine and a preparation method, and belongs to the technical field of biology. The method comprises the following steps: coupling nano magnetic beads with antibacterial peptide (polymyxin B sulfate), capturing Escherichia coli in a sample solution by using a nano magnetic bead compound solution modified by the antibacterial peptide, separating and resuspending the captured Escherichia coli from the sample solution under the action of an external magnetic field, and the like. The Escherichia coli captured through magnetic separation can be directly subjected to subsequent analysis; the method has the advantages of high enrichment efficiency, good specificity and low cost, does not need expensive instruments and equipment, and can be popularized and used in primary hospitals.
Owner:南昌大学第一附属医院
Analysis and detection method and application of polymyxin b sulfate
The invention discloses an analytical detection method of polymyxin sulfate B, comprising the steps of analytically detecting polymyxin sulfate B via high-performance liquid chromatography in combination with an evaporative light scatterer. The method allows four components in polymyxin sulfate B to be fully separated, has high specificity, is simple to perform, is free of disturbance by other organic impurities, is approximately effective to HPLC (high-performance liquid chromatography) of EP 9.0 bulk pharmaceutical chemicals in terms of high sensitivity, good simplicity and high speed, and allows the contents of components in a polymyxin sulfate B preparation to be measured more accurately.
Owner:SPH NO 1 BIOCHEM & PHARMA CO LTD
A kind of composite hydrogel wound dressing and preparation method thereof
ActiveCN111518288BEasy to prepareImprove antibacterial propertiesBandagesWound dressingCell migration
Owner:FUZHOU UNIV
Composite and freeze-dried powder containing polymyxin B sulfate, preparation methods thereof and application
ActiveCN111420027ADense and uniform structureImprove stabilityAntibacterial agentsPowder deliveryMicrobiologyPharmacology
The invention discloses a composite and freeze-dried powder containing polymyxin B sulfate, preparation methods thereof and application. The composite containing polymyxin B sulfate comprises the polymyxin B sulfate, tert-butanol and water; and on the premise of 1L of the composite containing polymyxin B sulfate in volume, the concentration of polymyxin B sulfate is 50-77g / L, and the concentrationof tert-butanol is 3-16 g / L. The preparation method of the freeze-dried powder containing polymyxin B sulfate comprises the step that the composite containing polymyxin B sulfate is subjected to freeze-drying treatment. The freeze-dried powder containing polymyxin B sulfate has a compact and even structure and has good stability and redissolution effect, and the preparation method comprises simple, convenient and easy processes.
Owner:SPH NO 1 BIOCHEM & PHARMA CO LTD
A kind of impurity of polymyxin b sulfate and preparation method thereof
ActiveCN109206486BQuality improvementEnsure safety and effectivenessPolymyxinsPeptide preparation methodsPhysical chemistryAnalytical chemistry
The invention discloses an impurity D17 of polymyxin B sulfate and a separation and preparation method thereof. The growth of the impurity under different conditions of temperature and pH is investigated. The preparation method provides a guarantee to control the content of the impurity, optimize the industrial production of the polymyxin B sulfate and improve the quality of a medicine.
Owner:HANGZHOU ZHONGMEI HUADONG PHARMA
Method for preparing colon targeted pill of polymyxin B sulfate used for poultry and livestock
InactiveCN1970076AGood treatment effectAntibacterial agentsPeptide/protein ingredientsTime lagMedicine
Owner:TIANJIN RINGPU BIO TECH
Method for screening and identifying antibiotic resistant bacteria
PendingCN114350738AEasy identificationProper selectionMicrobiological testing/measurementDNA/RNA fragmentationBiotechnologyResistant bacteria
The invention belongs to the technical field of bioengineering, and particularly relates to a method for screening and identifying antibiotic-resistant bacteria. According to the method, the soil suspensions with different concentration gradients are coated, and the optimal dilution gradient for effectively selecting single colonies is determined according to the condition of the colonies growing on a flat plate, so that subsequent large-batch screening and identification of antibiotic-resistant bacteria are facilitated; according to the method, the growth speed of the resistant bacteria is controlled by controlling the concentration of erythromycin, streptomycin and polymyxin B sulfate in the resistant culture medium, so that the purpose of accurately screening the drug-resistant bacteria is achieved; the sterilization gun head is used for replacing a traditional inoculating loop to select single colonies, the bacterium selecting step can be completed by repeated blowing and beating, and the problems that a large amount of time is consumed when the inoculating loop is burnt and cooled, the cooling degree is difficult to control, and bacteria are scalded to death are avoided.
Owner:INST OF URBAN ENVIRONMENT CHINESE ACAD OF SCI
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