The invention discloses a production method of cooling and keeping fresh beef. The production method of cooling fresh beef includes slaughtering, bleeding, peeling, and removing viscera. After the carcass passes the quarantine inspection, it is rinsed with water; The carcass is deboned, divided and trimmed, and then vacuum-packed, then hot-soaked and cold-soaked, and then placed in an environment of -2 to 2°C for storage. Compared with the prior art, the fresh-keeping beef obtained by the present invention has better safety and sanitation, and longer preservation period.