The invention provides a recombined porcine
interferon Gamma, a coding
gene thereof, and an expressing, purifying and inclusion body refolding method, and belongs to the field of biological
gene engineering. The recombined porcine
interferon Gamma serves as a non-specific broad-spectrum anti-viral biological preparation, and has a wide medicinal prospect in the field of veterinary medicines; but as most of
gene engineering veterinary medicines, the porcine
interferon Gamma has the problems of underproduction, high prices, non-
uniform quality and the like. According to the recombined porcine
interferon Gamma, the coding gene thereof and the expressing method, an
escherichia coli expression
system is used for performing
heterologous expression for the recombined porcine
interferon Gamma subjected to
codon optimization. Besides, aiming at the problem that the porcine
interferon Gamma in a
prokaryotic expression system is usually expressed in a manner of an inclusion body, the invention further provides the purifying and refolding method for the inclusion body of the recombined porcine interferon Gamma, so that the prepared recombined porcine interferon Gamma has
high activity and meets a standard for industrialized production.