The invention discloses an oxygen bottle fixing device for an ambulance, specifically relates to the field of first aid supplies, and mainly includes a shell, a side plate, a self-locking fixing frame, a tank seat, a sliding pressure frame and an oxygen bottle, and the middle part of the rear side of the side plate is fixed Installed with a fixed installation with a self-locking bracket. Compared with the existing technology, by setting the self-locking fixing frame, compared with the existing technology, the device can flexibly adapt to different vehicle interior heights, and can be flexibly and stably assembled with the vehicle without damage, and has strong applicability; by setting the sliding pressure Compared with the existing technology, this product can quickly carry out the labor-saving loading and unloading of the oxygen cylinder, convert the vibration impact into the flexible sliding of the spring and friction loss, and ensure the stability of the tank body, and the fixation is firm and the protection is perfect.