The invention discloses a preparation method of a pharmaceutical composition for killing 
beet armyworm. The preparation method comprises the following steps: 1) preparing raw materials; (2) adding water into radix stemonae, nidus vespae and fructus quisqualis, and decocting twice to obtain medicinal liquor for later use; 3) adding water into 
semen raphani, fennel, long-noded 
pit viper, radix flemingiae philippinensis, herba kummerowiae striatae and herba teucrii viscidi, decocting for twice, filtering, and concentrating the filtrate till the 
relative density is 1.15; 4) mixing the medicinal liquor; 5) regulating the pH value of the solution; 6) filtering, filling and sterilizing to obtain the pharmaceutical composition. The pharmaceutical composition disclosed by the invention can be usedfor specifically killing the 
beet armyworm, has the advantages of high safety, high biodegradability, freeness from 
pollution and pungent smell, freeness from environmental 
toxicity, no residues in animals, no damage to biological balance, low influence on the 
crop growing environment, higher permeability, higher moistening property, capabilities of interfering the 
central nervous system of the 
beet armyworm and restraining synthetic development of worm eggs, non-tendency to 
pesticide resistance and high fast-acting property, and is suitable for being widely popularized and applied on the aspect of pest prevention and control.