The invention discloses a preparation method of a medicinal composition for killing red spider mites, which comprises the following steps: 1) preparing raw materials; 2) decocting alum, veratrum, and pomegranate peel with water for two times, and the medicinal liquid for later use; 3) soap Add water to decoct the horned thorn, cumin, small Huanhun, Qingming willow, yarrow, and magnolia twice, filter, and concentrate the filtrate to a relative density of 1.15; 4) Merge the liquid medicine; 5) Adjust the pH value of the solution; 6) Filter, Filling, sterilization, that is. The pharmaceutical composition described in the present invention can kill spider mite in a targeted manner, has good safety, good biodegradation, no pollution and irritating odor, no environmental toxicity, no residue in animals, and no damage to ecological balance. It has little impact on the crop growth environment; it has good permeability and wetness, interferes with the nervous system of insects, inhibits the synthesis and development of insect eggs, and is not easy to develop drug resistance; it has good quick effect and is suitable for widespread application in pest control.