The invention relates to a sampler, in particular to a universal multisubstrate profile spiral injection type columnar sampler which is provided with a sampling tube, a handle, a rotary shaft, a locking ring, a toothed end surface, an end cover, a non-return blade and a spiral blade, wherein a scale for indicating sampling depth is carved on the inner wall of the sampling tube; the sampling tube consists of two half columns; the two half columns are fixed by the locking ring, one of the half columns is detachable, which is convenient for observing and collecting samples; the sampling tube is detachable and is provided with the toothed end surface; the handle is fixed to the top of the rotary shaft; the rotary shaft is arranged in the sampling tube; the end cover is arranged on the top of the sampling tube; the non-return blade is installed on the end of sampling tube; and the spiral blade is fixed below the non-return blade. By adopting an inner spiral injection type, the the sampler can be used for sampling under the multisubstrate conditions, such as mud, sand, detritus and the like., has the advantages of self-sealing function, rapid and convenient sampling, no leakage of samples, and easy separation of samples, sampling depth can be observed and profile samples can be efficiently obtained.