The invention discloses a skewer pulling machine, which comprises an L-shaped rack, a transferring mechanism, a clamping plate, a downward-pressing air cylinder part, a skewer pulling air cylinder, aclamping mechanism, an air supplying mechanism and a programmable controller. Beef jerky strings are sequentially placed onto the transferring mechanism at certain time intervals till being transferred onto the clamping plate for position limiting, a telescopic rod of the downward-pressing air cylinder part extends, the beef jerky strings are pressed through a pressing block, a telescopic rod of the skewer pulling air cylinder retracts, a sliding block slides on a guide rail to drive a clamping manipulator to move horizontally so as to clamp one ends of skewers, then the telescopic rod of theskewer pulling air cylinder extends, and then jerked beef and the skewers are separated. In the whole process, a proximity sensor, a first pressure sensor, a second pressure sensor, a first position sensor, a second position sensor and the programmable controller are utilized for controlling, continuous operation is achieved, the automation degree is high, the manual labor intensity is relieved, and the working efficiency is improved.