The application relates to the technical field of
gene detection, and discloses a method for detecting fusion genes based on
DNA-based
sequencing data, which comprises the following steps: step S1, acquiring
DNA sequencing data, filtering the
sequencing data by using
software fastp, and obtaining filtered sequencing data; step S2, detecting by using
software Genefuse, obtaining a first
fusion gene result and related parameters; step S3, detecting by using
software Factera, obtaining a second
fusion gene result and related parameters; step S4, detecting by using software Arriba, obtaining a third
fusion gene result and related parameters; and step S5, acquiring a fusion
gene filtering model, taking the first fusion
gene and related parameters, the second fusion gene and related parameters, and the third fusion gene and related parameters as input data to calculate a true positive fusion gene, and outputting a result. The application constructs a fusion
gene screening standard based on multiple software, avoids false positives or false negatives of a single software detection result, and improves the
detection rate and
correctness of the fusion gene.