The invention discloses a Fe-MOFs
chemiluminescence-
fluorescence double-response immunosensor. The immunosensor comprises detection antibodies and a paper
chip; the detection antibodies are labeled byFe-MOFs; the detection regions of the paper
chip are connected with capture antibodies; the non-specific sites of the paper
chip are sealed through a sealing agent; and the Fe-MOFs are a
metal organic frame material formed by Fe<3+> and 2-aminobenzene-1,4-
dicarboxylic acid. According to the immunosensor disclosed by the invention, the Fe-MOFs are used as
signal molecules, have relatively high stability and have relatively good water
solubility; and in addition, the material not only has fluorescent performance, but also can catalyze a luminal-H2O2 chemiluminiscence
system to generate chemiluminiscence. When the immunosensor disclosed by the invention is used for immunoreaction, two
modes of the
signal output of the detection result of the immunosensor can be provided, in particular, detection can be performed through either a
fluorescence method or a
chemiluminescence method, the two detection
modes can be flexibly selected, and therefore, detection
modes can be enriched, and the useof the two modes can further improve the accuracy of the detection result.