The invention discloses a Chinese medicine preparation for regulating weakness of spleen and stomach of people with deficiency of vital energy, a preparation method and application thereof. The main ingredients of the Chinese medicine preparation include the following Chinese medicinal herbs in parts by mass: 30-60 parts of Indian buead, 50-110 parts of semen nelumbinis, 45-90 parts of common yam rhizome, 50-100 parts of semen coicis, 50-110 parts of semen euryales, 20-50 parts of liquoric root, 50-110 parts of semen phaseoli, 25-50 parts of angelica root, 60-130 parts of orange peel, 30-70 parts of malt, 40-90 parts of fructus alpiniae oxyphyllae, 30-70 parts of fructus crataegi, 40-70 parts of Chinese radish seed, 50-80 parts of fructus amomi, 50-90 parts of folium nelumbinis, 30-60 parts of palmleaf raspberry fruit, 40-70 parts of glossy ganoderma and 50-80 parts of endothelium corneum gigeriae galli. The Chinese medicine preparation for regulating weakness of spleen and stomach of people with deficiency of vital energy, provided by the invention, is prepared by adopting medicinal and eatable plants, has no any side effects, and can effectively relieve or eliminate adverse symptoms that people suffering from deficiency of vital energy are easy to cause short of breath and dry skin, usually feel hand and foot center heating, do not like talking, speak with low volume, are easy to catch a cold, suffer from usual abnormal sweating due to general debility, and usually feel tired and the like.