The invention relates to an organic molecular line. The molecular line comprises tetrathiafulvalene, derivative thereof and a substrate, the tetrathiafulvalene and the derivative thereof are accumulated along the lowest-system energy direction Pi-Pi on the surface of the substrate, the intermolecular distance of the organic molecular line is 0.5nm to 0.8nm, and the organic molecular line is 40nm to 400nm long and 0.8nm to 1.2nm wide; the organic molecular line has a single-layered double-molecular line structure or a single-line structure on the surface of the substrate; and the molecular line makes the included angle of 60 degrees or 120 degrees with the molecular axis. A method for preparing the molecular line has the following steps that: TTFs is dispersed and dissolved in tetradecane,so that the tetradecane solution of TTFs is obtained; the tetradecane solution of TTFs is dripped to a clean, even highly oriented pyrolytic graphite (HOPG) surface, and after the solution is dispersed and stabilized on the surface, the one-dimensional ordered TTFs molecular line is obtained. The invention utilizes the solution method to realize the self-assembling growth of TTFs molecules on thesolid surface, a molecular line which is tens to hundreds of nanometers long can be obtained, and the preparation technique is simple, and is applicable to various TTFs molecules.