A steam turbine rotor anti-fatigue life partition control method, the method is the following steps: (1), start the steam turbine unit of the power plant, record the structural size and operating parameters of the steam turbine rotor; (2), finite element modeling, Thermal calculations and simulation calculations under variable working conditions; (3), peak stress calculations in different temperature zones, and material fatigue characteristics analysis; (4), formulating rush rotation, medium-speed warm-up, constant-speed warm-up, grid-connected low-load warm-up, Six-stage optimized operation control plan for cutting cylinders and entering sliding pressure operation; (5) Draw optimized start-up curves, and organize construction personnel to operate the start-up and operation of steam turbine units on site. The invention reasonably distributes the anti-fatigue life loss, optimizes the change rate of operating parameters under variable working conditions, shortens the start-up time, ensures the economy of the start-up process, and meets the flexibility requirements of the peak-shaving demand of the power grid.