Using pulsed-wave (PW) ultrasonography for determining an aliasing-free radial velocity spectrum of matter moving in a region. Includes: transmitting into the region a plurality of pulse trains of sound waves at two or more different pulse repetition frequencies; spectrally analyzing each pulse train, for evaluating a Doppler frequency spectrum associated with each pulse train; combining frequency components of Doppler frequency spectrum of each pulse train, for obtaining aliasing-free instantaneous Doppler frequency spectrum for the region; using Doppler effect for translating aliasing-free frequency spectrum to aliasing-free radial velocity spectrum. Implementable using pulsed-wave Doppler (PWD), color flow Doppler (CFD), tissue Doppler imaging (TDI), or pulsed-wave (PW) ultrasonography. Applicable to liquid or/and solid forms of matter, moving in a two- or three-dimensional region. Matter is any substance or material, composed of organics or/and inorganics, which is part of a non-living object, or, part of a human or animal subject.